环境内分泌干扰物2—溴丙烷对蝾螈精原细胞毒性的研究

目的探讨2-溴丙烷对精原细胞的毒性;建立蝾螈精原细胞离体培养系统用于检测化学物的生殖毒性.方法运用蝾螈离体精原细胞培养及整体染毒实验,研究2-溴丙烷对精原细胞的形态学、死亡率和增殖率的影响.离体染毒浓度为0.01、0.1、1mmol/L,以FSH为阳性对照,L-15培养液为空白对照.整体实验给蝾螈一次性腹腔注射,剂量为0、200、600、1800mg/kg.结果不论是离体培养实验还是整体实验,随着2-溴丙烷染毒浓度的增高,精原细胞的增殖率下降、死亡率上升(P＜0.05),且呈剂量反应关系.组织学结果显示,2-溴丙烷在影响蝾螈其他脏器之前,先损害睾丸的精原细胞.精原细胞出现核固缩、细胞坏死.结论精原细胞可能是2-溴丙烷的靶细胞,蝾螈离体精原细胞培养系统可作为测定或筛选外来化学物生殖毒性的一种辅助手段.

Study on newt spermatogonia toxicity of 2-bromopropane, an environmentalendocrine disrupter

Objective This study was to explore the toxicity of 2-bromopropane on spermatogonia,establish the newt spermatogonia culture system in vitro as a system to detect the reproductive toxicity of exogenous chemicals.Methods Male adult newts selected as the animal model.Both in vitro and in vivo experiments were conducted to study the effects of 2-bromopropane on the morphology,death rate and proliferation rate of spermatogonia.In vitro,newts were divided into 5 groups,i.e.0.01,0.1 or 1 mmol/L 2-bromopropane,FSH positive control and L-15 negative control group.In vivo,newts were administrated (i.p.)2-bromopropane at doses of 0,200,600 or 1 800 mg/kg.Results Both in vitro and in vivo experiments showed that the death rate of spermatogonia rose while the proliferation rate dropped significantly( P <0.05) with the increase of 2-bromopropane concentration.Histological examination showed that 2-bromopropane harmed spermatogonia before it exerted any toxic effect on other organs.Some of spermatogonia had pyknotic nucleus and some appeared necrosis.Conclusion Spermatogonia may be the target cell of 2-bromopropane's reproductive toxicity.In vitro newt spermatogonia culture system may serve as an accessory method for detecting or screening the reproductive toxicity of exogenous chemicals including environmental endocrine disrupters.