宁夏产柴胡药材与其对照药材的HPLC指纹图谱及近红外光谱比较

目的:通过宁夏13批柴胡药材与对照药材的HPLC指纹图谱和近红外图谱研究,对宁夏柴胡药材进行质量评价。方法:分别建立HPLC指纹图谱和近红外光谱,比较13批柴胡药材与对照药材HPLC和近红外光谱的相似度,并分别对13批柴胡药材和对照药材的共有峰面积和近红外吸收进行配对样本t检验。结果:13批柴胡药材与对照药材色谱指纹图谱和近红外图谱的相似度分别0.901和0.982,结合13批柴胡药材与对照药材共有峰面积(近红外为对应点吸收)的配对样本t检验验证,均无显著差异,验证了13批柴胡药材与对照药材质量的一致性较高。结论:本研究基于色谱指纹图谱与近红外图谱,并结合配对样本t检验对分析结果进行验证,认为13批宁夏产柴胡药材与对照药材质量无显著差异。研究采用两种综合性较强的检测手段,避免了分析中因指纹图谱相似度阈值限定不明确而引起的误判,为分析结果提供了可靠的依据,为完善中药材质量控制方法提供了新的思路。

Comparative Study on HPLC Finger-print and Near-infrared Spectra of Bupleuri Radix Produced in Ningxia and Its Control Medicinal Herb

Objective: To evaluate the quality of Bupleuri Radix produced in Ningxia by HPLC finger-print and near-infrared spectrum for 13 batches of Bupleuri Radix and its control medicinal herb. Method: HPLC finger-print and near-infrared spectroscopy were built up for comparing the similarities between the 13 batches of Bupleuri Radix and its control medicinal herb, respectively. Furthermore, the paired t-test was carried out to analyze common peak areas and near-infrared spectrum between the 13 batches of Bupleuri Radix and control medicinal herb, respectively. Result: The results showed that the similarities of HPLC finger-print and near-infrared spectrum for the 13 batches of Bupleuri Radix and control medicinal herb were more than 0.901 and 0.982, respectively. Moreover, paired sample t-test showed no significant difference between common peak areas of the 13 batches of Bupleuri Radix produced in Ningxia and control medicinal herb. This verified a high consistency between the 13 batches of Bupleuri Radix and control medicinal herb. Conclusion: On the basis of methods of chromatographic finger-print and near-infrared spectrum, this study applied paired sample t-test to verify analysis results, which suggested no significant difference between the 13 batches of Bupleuri Radix produced on Ningxia and control medicinal herb. The study used two comprehensive detection means, which could avoid erroneous judgment caused by uncertain threshold values of similar degree in the chromatographic finger-print and provide a reliable basis for the analysis results and new ideas for improving the quality control method of traditional Chinese medicinal herbs.