深静脉血栓形成对大鼠远隔静脉血管内皮细胞凋亡的影响

目的：探讨深静脉血栓对非血栓段静脉内皮细胞的损伤作用。方法：90只SD大鼠随机分为血栓组、手术对照组和正常对照组。血栓组在左髂静脉以结扎法建立血栓模型；手术对照组开腹显露髂动静脉模拟同等程度创伤, 但不做静脉分离和结扎；正常组直接取材。取材标本是未形成血栓的上腔静脉和髂静脉及其酶解下的内皮细胞, 送电镜、流式细胞仪凋亡检查。结果：血栓组非血栓段内皮细胞形态与手术对照组均有明显改变；血栓组与手术对照比较, 1, 2, 4, 7 d 术后早期凋亡率差异均有统计学意义(均P＜0.05, P＜0.01）。手术对照组早、晚期调亡率与正常组比较, 也有显著差异, 但较血栓组程度低, 持续时间较短；手术对照组建模后4, 7d与正常组比较, 晚期凋亡率无统计学意义。结论：局部深静脉血栓可以导致其他远隔部位静脉内皮细胞损伤, 易发生早期凋亡。

Effects of deep venous thrombosis on apoptosis of endothelial cells of distant vein segment in rats

Objective To search the effect of venous thrombosis(VT) on the endothelial cells(ECs) of non-thrombosis vein segment(ECNT).Methods Ninty rats were divided into a normal group,a thrombosis group established by ligating a section of iliac vein,and a sham operation control group.An electroscope was used to detect the ECs of target vessels morphological changes.The Ecs were collected from non-thrombotic superior vena cava and iliac veins by enzymolysis and studied by flow cytometer to detect the positive rate o...