| SELDI技术筛选肺癌患者血清标志蛋白的临床价值 |
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| 引用本文： | 周继红,柳广南,黄斯明,钟小宁,苏红. SELDI技术筛选肺癌患者血清标志蛋白的临床价值[J]. 山东医药, 2010, 50(5): 18-19 |
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| 作者姓名： | 周继红 柳广南 黄斯明 钟小宁 苏红 |
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| 作者单位： | 广西医科大学第一附属医院,南宁,530021 |
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| 基金项目： | 广西自然科学基金项目 |
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| 摘 要： | 目的探讨表面增强激光解析电离飞行时间质谱（SELDI-TOF-MS）技术筛选肺癌患者血清标志蛋白的临床价值。方法用SELDI-TOF-MS技术、弱阳离子交换蛋白芯片,检测肺癌和肺良性病变患者的血清蛋白质质谱图;用Biomarker Pattern软件分析肺癌差异蛋白并初建其诊断模型,通过盲筛验证诊断模型。结果发现有统计学差异的蛋白峰20个,其中肺癌患者血清高表达蛋白质波峰14个,低表达蛋白质波峰6个;用质荷比2 090.77、2 503.31 Da的差异蛋白峰建立分类树模型,其诊断肺癌的灵敏度88%,特异度95%;盲筛验证灵敏度90%,特异度100%,粗符合率93.33%,Youden指数0.9。结论SELDI-TOF-MS技术筛选的肺癌血清差异性蛋白及分类树模型,诊断肺癌的灵敏度高、特异性好。
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| 关 键 词： | 表面增强激光解析电离飞行时间质谱技术 肺肿瘤 标志蛋白,血清 |
Clinical value of sera protein markers by surface-enhanced laser desorption ionization time of flight mass spectrometry in patients with lung cancer |
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| ZHOU Ji-hong,LIU Guang-nan,HUANG Si-ming,ZHONG Xiao-ning,SU Hong. Clinical value of sera protein markers by surface-enhanced laser desorption ionization time of flight mass spectrometry in patients with lung cancer[J]. Shandong Medical Journal, 2010, 50(5): 18-19 |
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| Authors: | ZHOU Ji-hong LIU Guang-nan HUANG Si-ming ZHONG Xiao-ning SU Hong |
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| Affiliation: | ZHOU Ji-hong,LIU Guang-nan,HUANG Si-ming,ZHONG Xiao-ning,SU Hong (First Affiliated Hospital of Guangxi Medical University,Nanning 530021,P.R.China) |
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| Abstract: | Objective To detect the clinical value of the protein markers in sera of the patients with lung cancer by surface-enhanced laser desorption ionization time of flight mass spectrometry(SELDI-TOF-MS) technology.Methods SELDI-TOF-MS technology and protein chips weak cation exchange(WCX-2 chip) were used to detect the protein mass spectrum in sera of patients with lung cancer and patients with benign pulmonary diseases.The different protein markers were analyzed by biomarker pattern software and the initial dia... |
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| Keywords: | SELDI-TOF-MS lung cancer protein markers serum |
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