On the mechanism of insulinotropic action of hepoxylins: Evidence of a direct,glucose-independent effect of hepoxylins B3 on insulin secretion |
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Authors: | V P Fedotov N V Sadovnikova I N Baranova L L Vasil'eva T A Manukina K K Pivnitskii |
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Institution: | (1) Institute of Experimental Endocrinology, Endocrinology Research Center, Russian Academy of Medical Sciences, Moscow |
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Abstract: | The effects of lipoxygenase metabolites of arachidonic acid, hepoxylin B3 epimers, on insulin secretion by a culture of isolated islet cells were studied. The effect was assessed at hepoxylin B3 concentrations of 0.2 to 5.0 μM and different glucose concentrations in the culture medium. Both hepoxylin B3 epimers were shown to boost the stimulating effect of glucose on insulin secretion. This effect manifests itself at glucose
concentrations of 5.5 and 11 mM and disappears at an above normal glucose content in the medium (20 mM). The capacity of hepoxylin
B3 to stimulate the secretion of insulin by a culture of islet cells in a glucose-free medium has also been demonstrated. This
direct, not glucose-mediated, insulinotropic effect may serve as proof that the hepoxylins belong to the category of intracellular
messengers.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N
o
10, pp. 404–406, October, 1995
Presented by I. G. Akmaev, Member of the Russian Academy of Medical Sciences |
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Keywords: | eicosanoids hepoxylins pancreatic islet cell culture insulin secretion |
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