Iron intake, red cell indicators of iron status, and DNA damage in young subjects |
| |
Authors: | Daniel Prá ,Angelica Bortoluzzi,Luiza Louzada Mü ller,Liziane Hermes,Jorge André Horta,Sharbel Weidner Maluf,Joã o Antonio Pê gas Henriques,Michael Fenech,Silvia Isabel Rech Franke |
| |
Affiliation: | a PPG em Promoção da Saúde, Universidade de Santa Cruz do Sul, Santa Cruz do Sul, Río Grande do Sul, Brazil b Nutrigenomics and Genome Health Laboratory, CSIRO Human Nutrition, Adelaide, SA, Australia c PPG em Saúde e Comportamento, Universidade Católica de Pelotas, Pelotas, Rió Grande do Sul, Brazil d Curso de Nutrição/DEDFIS, Universidade de Santa Cruz do Sul, Santa Cruz do Sul, Río Grande do Sul, Brazil e Serviço de Genética Médica, Hospital de Clínicas de Porto Alegre, Porto Alegre, Río Grande do Sul, Brazil f Instituto de Biotecnologia, Centro de Ciências Biológicas e da Saúde, Universidade de Caxias do Sul, Rio Grande do Sul, Brazil |
| |
Abstract: | ObjectiveThis study evaluated the association between primary DNA damage and chromosomal damage with iron intake and red blood cell parameters of iron status in a sample of healthy children and adolescents from a low-socioeconomic community.MethodsThe level of primary DNA damage was assessed using an alkaline comet assay and the level of chromosomal damage was assessed using the cytokinesis-block micronucleus assay. A automated complete blood count was used to evaluate red blood cell status. The intake of iron was measured using a food-recall questionnaire.ResultsAccording to hemoglobin levels, only 1 of the 30 subjects evaluated was anemic. Nevertheless, 43% of the sampled subjects showed decreased mean corpuscular volume in addition to an increased amount of primary DNA damage (P < 0.05). Mean corpuscular volume was negatively correlated with primary DNA damage (r = −0.429, P = 0.020) but not with chromosomal damage. The association between iron and primary DNA damage showed a U-shaped curve, indicating that an intake of approximately 15 mg of iron per day (up to two-fold of the dietary recommended intake) could minimize primary DNA damage in this age group. The frequency of micronuclei and nucleoplasmic bridges, indicators of chromosomal breakage/loss and chromosomal end-fusions, respectively, showed a negative correlation with iron intake. These results indicate that an intake of iron >15 mg/d could increase genomic stability in binucleated lymphocytes of the same group.ConclusionAn intake of iron ≥15 mg/d can decrease DNA damage in young subjects. |
| |
Keywords: | DNA damage Micronucleus Anemia Iron Micronutrients |
本文献已被 ScienceDirect 等数据库收录! |
|