被动运动和电刺激对尾吊大鼠比目鱼肌肌纤维的影响

目的观察被动运动、电刺激对尾吊大白鼠比目鱼肌各型肌纤维横截面积及肌纤维构成比的影响。方法72只发育期Wistar品系雄性大白鼠(体重68～76g)，随机分为对照组3组，模型组2组，被动运动组2组，电刺激组2组，每组8只。采用SDH染色，结合微机图像分析技术，对比目鱼肌肌纤维横截面积和肌纤维构成比进行定量分析。结果被动运动组、电刺激组比目鱼肌各型肌纤维面积与模型组比较有所增加；悬吊2周后，模型组比目鱼肌红肌纤维比例下降，中间肌纤维比例上升。结论被动运动、电刺激具有减缓尾吊大白鼠比目鱼肌废用性肌萎缩的作用。

The effects of passive movement and electrical stimulation on soleus of the rats treated with tail suspension

Objective To study the effects of passive movement (PM) and electric stimulation(ES) on soleus of the tail suspended rats. MethodsSeventy-two male Wistar rats(68~76g) were used in this experiment and divided into a normal control group, a model group (tail suspension), a ES group (tail suspension plus ES) and a PM group(tail suspension plus PM).The cross-sectional area of the muscle fibers and proportion of various types of muscle fibers in soleus were observed by histochemical staining of succinic dehydrogenase (SDH) and image pattern analyzer. ResultsThe results showed that the cross-sectional area of all types of soleus muscle fibers in the PM and ES groups was significantly larger than that in the model group, with the area of the intermediate muscle fibers more significantly larger in particular. The proportion of the red fibers decreased and that of the intermediate fibers increased in the model group after one week of tail-suspension. ConclusionPassive movement and electrical stimulation could slow down the muscle atrophy of soleus of the tail-suspended rats.