| 分泌抗人早期T细胞单克隆抗体杂交瘤细胞株的建立及初步鉴定 |
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| 引用本文： | 宋强,张明珙,宋素芹,李杰,赵川莉,郭承山,王欣.分泌抗人早期T细胞单克隆抗体杂交瘤细胞株的建立及初步鉴定[J].山东大学学报(医学版),1996(3). |
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| 作者姓名： | 宋强 张明珙 宋素芹 李杰 赵川莉 郭承山 王欣 |
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| 作者单位： | 山东医科大学附属医院血液病研究室 |
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| 摘 要： | 以胎儿胸腺细胞免疫ＢＡＬＢ／ｃ小鼠，用Ｂ淋巴细胞杂交瘤技术产生了两种单克隆抗体，分别命名为ＳＭＵ１３、ＳＭＵ１４。初步鉴定的结果表明，（１）ＳＭＵ１３、ＳＭＵ１４是抗早期Ｔ淋巴细胞的单克隆抗体，但与浆细胞也发生反应；（２）两株单抗的特性、反应谱与ＣＤ３８抗原相似；（３）ＳＭＵ１３、ＳＭＵ１４在白血病免疫学分型中可以明确判断白血病细胞的来源，具有结合补体的能力，在急性Ｔ淋巴细胞白血病的治疗中可发挥重要作用
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| 关 键 词： | 抗体，单克隆 杂交瘤 白血病 抗原，表面 |
THE PRODUCTION AND PRELIMINARY IDENTIFICATION OF HYBRID CELL LINES SECRETING MONOCLONAL ANTIBODIES AGAINST HUMAN PRE-T LYMPHOCYTES |
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| Song Qiang ,Zhang Minggong, Song Suqin,et al.THE PRODUCTION AND PRELIMINARY IDENTIFICATION OF HYBRID CELL LINES SECRETING MONOCLONAL ANTIBODIES AGAINST HUMAN PRE-T LYMPHOCYTES[J].Journal of Shandong University:Health Sciences,1996(3). |
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| Authors: | Song Qiang Zhang Minggong Song Suqin |
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| Abstract: | Two monoclonal antibodies SMU13 and SMU14 were produced by fusing SP2/0 murine myeloma cells and BALB/c murine spleen cells immunized with thymus cells. A preliminary identification of the antibodies indicated that: (1)SMU13 and SMU14 are antibodies against pre-T lymphocytes and plasma cells; (2) their characteristics and reactivity spectrum are similiar to those of the CD38 antigen; (3) in the analysis of leukemia immunophenotype, SMU13 and SMU14 can give a better classification for the origin of leukemic cells and may have therapeutic application for the treatment of T-ALL because of their capability of combining with complements. |
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| Keywords: | Antibodies monoclonal Hybridomas Leukemia Antigens surface |
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