红腺忍冬叶不同溶剂提取物中葡萄糖和果糖的含量变化研究

目的]建立用HPLC-ELSD法测定红腺忍冬叶提取物中葡萄糖和果糖含量的方法，并应用此方法研究红腺忍冬叶不同溶剂提取物中葡萄糖和果糖含量的变化。方法]采用Lichrospher NH2色谱柱，乙腈-水（90：10）为流动相，柱温25℃，流速0.8mL/min；ELSD漂移管温度70℃，载气为氮气，流速1.6L/min。结果]红腺忍冬叶提取物中的葡萄糖和果糖能够得到较好地分离，葡萄糖和果糖的线性范围分别为0.5840~1.168mg·mL-1、0.8256~1.8576mg·mL-1，r分别为0.9994、0.9991；平均加样回收率分别为96.88%、97.55%，RSD分别为1.11%、1.15%；葡萄糖和果糖的稳定性（RSD）分别为2.12%、0.38%，重复性（RSD）分别为1.31%、0.24%。其中，水提物中葡萄糖和果糖的含量最高（分别为2.19mg·mL-1、4.10mg·mL-1），50%醇提物次之（含量分别为1.13mg·mL-1、2.12mg·mL-1），95%醇提物中含量最低（分别为0.45mg·mL-1、0.65mg·mL-1）。结论]该方法简单、准确、重复性好，可用于测定红腺忍冬叶提取物中葡萄糖和果糖的含量。该批次红腺忍冬叶中，葡萄糖和果糖含量为水提液〉50%乙醇提取物〉95%乙醇提取物。

Study on the Content Change of Glucose and Fructose in Different Solvents Extracts of Leaves of Lonicera Hypoglauca Miq.

Objective]To establish a method to determine the contents of glucase and fructose in extracts of leaves of Lonicera hypoglauca Miq. using HPLC-ELSD, and apply this method to study the content change of glucose and fructose in different solvents extracts in leaves of Lonicera hypoglauca Miq. Methods] The chromatographic separation was achieved on Lichrospher NH2 column using a mobile phase composed of a mixture of acetonitrile and water（90：10） at a flow rate of 0.8mL·min-1 and the column temperature was 25℃.The ELSD drift tube temperature was set at 70℃ and the carrying gas was N2 and the flow rate of the gas was 1.6L·min-1. Results] Glucose and fructose from extracts of the leaves of Lonicera hypoglauca Miq. could be wel separated, and the linearity ranges of glucose and fructose were shown at the concentration of 0.5840~1.168mg·mL-1 and 0.8256~1.8576mg·mL-1, the average recovery rate of glucose and fructose were 96.88%（RSD=1.11%） and 97.55%（RSD=1.15%）, the stability of glucose and fructose was 2.12%and 0.38%, and the repeatability was 1.31% and 0.24%. Among the water extract,50% alcohol extract and 95% alcohol extract,the content of the glucose and fructose in the water extract was highest,which was 2.19mg·mL and 4.10mg·mL-1, the 50% alcohol extract was second, which was 1.13mg·mL-1 and 2.12mg·mL-1, and the 95% alcohol extract was lowest, which was 0.45mg·mL-1 and 0.65mg·mL-1.Conclusion] The method is simple, accurate and has good repeatability, and can be used to determine the contents of glucose and fructose in the extract of leaves of Lonicera hypoglauca Miq. In this batch of leaves of Lonicera hypoglauca Miq., the contents of fructose and glucose were of water extract〉50%ethanol extract〉95%ethanol extract.