| CHANGES IN EXPRESSION AND STRUCTURE OF SOME PROTOONCOGENES IN TPA-INDUCED HL-60 AND RAJI CELLS |
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| 作者姓名： | 唐建清 陈诗书 |
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| 作者单位： | Department of Biochemistry，Laboratory of Molecular Biology，SSMU，Shanghai，Department of Biochemistry，Laboratory of Molecular Biology，SSMU，Shanghai |
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| 摘 要： | Different expression levels of c-myc, c-H-ras, c-sis and c-erb were observed in HL-60 and Raji cells. Induction of HL-60 cells with TPA (10ng/ml) for two days elicited differentiation together with a decreased expression level of c-myc and c-H-ras. No significant changes in morphological structure in Raji cells were seen to occur over a 4-day induction period with TPA (1ng/ml), but the level of expression of c-H-ras declined slightly over the period whereas that of c-myc remained unchanged. From the EcoRI and SacI restriction fragment patterns, it can be seen that the c-myc oncogene was amplified in HL-60 and different in structure in Raji as compared to WBC. After induction the patterns of the two restriction endonuclease fragments for HL-60 cells showed no change. The pattern of Sacl fragments of c-H-ras gene of Raji cells induced with TPA for 4 days displayed an additional fragment of 12.7 Kb. Possible roles of oncogene expression in cell differentiation are discussed.
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CHANGES IN EXPRESSION AND STRUCTURE OF SOME PROTOONCOGENES IN TPA-INDUCED HL-60 AND RAJI CELLS |
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| Tang Jianqing,Chen Shishu.CHANGES IN EXPRESSION AND STRUCTURE OF SOME PROTOONCOGENES IN TPA-INDUCED HL-60 AND RAJI CELLS[J].Journal of Shanghai Second Medical University(Foreign Language Edition),1988(2). |
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| Authors: | Tang Jianqing Chen Shishu |
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| Institution: | Tang Jianqing,Chen Shishu Department of Biochemistry,Laboratory of Molecular Biology,SSMU,Shanghai |
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| Abstract: | Different expression levels of c-myc, c-H-ras, c-sis and c-erb were observed in HL-60 and Raji cells. Induction of HL-60 cells with TPA (10ng/ml) for two days elicited differentiation together with a decreased expression level of c-myc and c-H-ras. No significant changes in morphological structure in Raji cells were seen to occur over a 4-day induction period with TPA (1ng/ml), but the level of expression of c-H-ras declined slightly over the period whereas that of c-myc remained unchanged. From the EcoRI and SacI restriction fragment patterns, it can be seen that the c-myc oncogene was amplified in HL-60 and different in structure in Raji as compared to WBC. After induction the patterns of the two restriction endonuclease fragments for HL-60 cells showed no change. The pattern of Sacl fragments of c-H-ras gene of Raji cells induced with TPA for 4 days displayed an additional fragment of 12.7 Kb. Possible roles of oncogene expression in cell differentiation are discussed. |
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| Keywords: | oncogene lymphoma leukemia phorbol esters cell defferentiation |
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