| 弗氏柠檬酸杆菌随机扩增多态性DNA法基因分型 |
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| 引用本文： | 叶明亮,黄象艳,吕波,侯晓琦,李银太.弗氏柠檬酸杆菌随机扩增多态性DNA法基因分型[J].中华医院感染学杂志,2005,15(10):1107-1109. |
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| 作者姓名： | 叶明亮 黄象艳 吕波 侯晓琦 李银太 |
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| 作者单位： | 1. 济南军区总医院,山东,济南,250031
2. 军事医学科学院野战输血研究所,北京,100850 |
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| 摘 要： | 目的建立弗氏柠檬酸杆菌(Citrobacter freundii)随机扩增多态性DNA(RAPD)法基因分型图谱. 方法以优化的反应体系及单一随机引物L8,对临床分离的116株C.freundii进行RAPD分析,并按指纹图上DNA条带数及片段大小绘制基因分型图谱. 结果 116株临床分离C.freundii共得78种RAPD型. 结论 RAPD法可将C.freundii分型78种.
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| 关 键 词： | 弗氏柠檬酸杆菌 随机扩增多态性DNA 基因分型 |
| 文章编号： | 1005-4529（2005）10-1107-03 |
| 收稿时间： | 2004-11-18 |
| 修稿时间： | 2005-02-10 |
Genotyping of Citrobacter freundii by Randomly Amplified Polymorphic DNA Technique |
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| YE Ming-liang,HUANG Xiang-yan,L Bo,HOU Xiao-qi,LI Yin-tai.Genotyping of Citrobacter freundii by Randomly Amplified Polymorphic DNA Technique[J].Chinese Journal of Nosocomiology,2005,15(10):1107-1109. |
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| Authors: | YE Ming-liang HUANG Xiang-yan L Bo HOU Xiao-qi LI Yin-tai |
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| Institution: | General Hospital, Jinan Military Command, Jinan 250031, China |
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| Abstract: | OBJECTIVE To establish the genotype profiles of Citrobacter freundii by randomly amplified polymorphic DNA(RAPD) technique.METHODS The optimal reaction system and single random primer L8 were used for genotyping of the 116 strains of C.freundii isolated from clinical samples,and the fingerprinting profiles were drawn according to the numbers and lengths of DNA bands.RESULTS The 116 strains of C.freundii isolated from clinical samples could be distinguished to 78 kinds of RAPD patterns.CONCLUSIONS Seventy eight kinds of genotype profiles could be obtained by RAPD technique in the C.freundii isolated from clinical samples. |
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| Keywords: | Citrobacter freundii Randomly amplified polymorphic DNA Genotyping |
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