| 定向诱导的骨髓基质细胞移植后在大鼠缺血脑组织中的分布 |
| |
| 引用本文： | 庞克军,涂伟,邓志锋,汪泱,李东海,娄远蕾.定向诱导的骨髓基质细胞移植后在大鼠缺血脑组织中的分布[J].江西医学检验,2008(4):366-368. |
| |
| 作者姓名： | 庞克军 涂伟 邓志锋 汪泱 李东海 娄远蕾 |
| |
| 作者单位： | 南昌大学第二附属医院神经外科;南昌大学第一附属医院泌尿外科研究所; |
| |
| 基金项目： | 国家自然科学基金资助项目(No.30560156); 江西省自然科学基金资助项目(No.2007gzy1187) |
| |
| 摘 要： | 目的探讨人骨髓基质细胞（bone marrow stromal cells，MSCs）源神经细胞体内移植后的分布状况。方法采用密度梯度离心结合贴壁分离法分离、纯化人MSCs。MSCs经bFGF预诱导24h及阿魏酸钠（Sodium Ferulate）诱导24h后用于移植。采用线栓法复制大鼠局灶性脑缺血再灌注动物模型．缺血2h后行再灌注，24h后经栓塞侧颈内动脉注入诱导后的MSCs（5×10^6/0．8ml）（n=61。2w后取脑组织行冰冻切片．通过检测人细胞核特异抗体MAB1281来鉴定移植的人MSCs。结果MSCs经阿魏酸钠诱导后．细胞逐渐圆缩并伸出突触．呈现神经细胞样形态。在移植大鼠脑内可检测到MAB1281呈阳性表达的人MSCs．且MSCs主要分布在缺血侧损伤灶的周围，明显高于对侧同部位及其它脑区。结论人MSCs源神经细胞移植后在大鼠脑内存活并主要聚集在脑内缺血灶的周围。
|
| 关 键 词： | 骨髓基质细胞 阿魏酸钠 诱导 移植 |
Study of transplantion of human bone marrow stromal cells after orientiatedly induced into neuron-like cells |
| |
| PANG Kejun,TU Wei,DENG Zhifeng,et al..Study of transplantion of human bone marrow stromal cells after orientiatedly induced into neuron-like cells[J].Jiangxi Journal of Medical Laboratory Sciences,2008(4):366-368. |
| |
| Authors: | PANG Kejun TU Wei DENG Zhifeng |
| |
| Institution: | PANG Kejun,TU Wei,DENG Zhifeng,et al. Department of Neurosurgery,The Second Affiliated Hospital of Nanchang University,Nanchang 330006,China |
| |
| Abstract: | Objective To investigate the distribution of human bone marrow stromal cells(MSCs) transplanted into rat brain after induced into neuron-like cells. Methods MSCs were isolated by gradient centrifugation in lymphocyte separation medium and by their adher- ence to tissue culture surfaces. The cultured MSCs were induced with sodium ferulate (SF) for 24 hours after pre-induced by bFGF for 24 hours. The right middle cerebral artery occlusion (MCAO) model of SD rats was produced by obstructing MCA using a single ... |
| |
| Keywords: | Bone marrow stromal cell Sodium ferulate Induction Transplantation |
| 本文献已被 CNKI 维普 等数据库收录！ |
