紫外线C对A549细胞HSPs表达的影响

目的]研究紫外线C(UVC)对热休克蛋白(heat shock proteins,HSP)27、70及90的影响。方法]用紫外线不同照射剂量(0、10、20、40、80J/m2)来照射A549细胞株,用Western-blot检测HSP27、HSP70、HSP90的表达水平。结果]随着UVC剂量的升高,HSP70和HSP27的表达水平先升高,达到一个高峰后又降低。HSP27表达在20J/m2时达到高峰,HSP70表达在40J/m2时达到高峰,HSP90各剂量的UVC照射下,未见显著性变化。与对照组(0J/m2)相比,在10J/m2时,HSP27和HSP70表达增加(P<0.05);在20J/m2时,HSP27表达显著增加(P<0.01),HSP70表达也继续增加(P<0.05);在40J/m2时,HSP27表达开始下降,但与对照组相比差异没有显著性(P>0.05),HSP70表达仍显著增加,与对照组相比差异有显著性(P<0.01)。在80J/m2时,HSP27表达与对照组和40J/m2组相比,差异无显著性(P>0.05),HSP70表达下降,但仍高于对照组(P<0.05)。在10、20、40和80J/m2的UVC照射下,HSP90表达变化均未见显著性差异(P>0.05)。结论]随着UVC剂量的升高,HSP70和HSP27的表达水平先升高,达到一个高峰后又降低。HSP27,HSP70能被一定剂量范围的UVC照射所诱导,高剂量UVC照射时HSP27、HSP70的表达被抑制,但HSP90表达未发现能被诱导。

Effect on Heat Shock Proteins Expression in A549 Cells Induced by Ultraviolet C Irradiation

Objective] To explore the expression of HSP27,HSP70,and HSP90 in A549 cells exposed to different Ultraviolet C(UVC)irradiation. Methods] A549 cells were exposed to 10,20,40,and 80 J/m2 Ultraviolet C irradiation respectively,with normal cultured cells as a control. Expression of HSP27,HSP70 and HSP90 was detected by western-blot assay. Results] Along with the increasing dosage of UVC irradiation,HSP27 and HSP70 levels increased to a peak then decreased. HSP27 level rose up to a peak under 20 J/m2 of UVC irradiation but HSP70 level rose up to a peak under 40 J/m2 of UVC irradiation. Compared with the group of 0 J/m2,HSP70 and HSP27 levels increased significantly in the group of 10 J/m(2P<0.05)and HSP27 increased very significantly in the group of 20 J/m(2P<0.01). HSP70 levels increased obviously in the group of 40 J/m(2P<0.01) but HSP27 level reduced to normal leve(lP>0.05). In the group of 80 J/m2,HSP70 levels decreased significantly compared with 40 J/m2(P<0.05)but still higher compared with the group of 0 J/m2(P<0.05). Significant changes of HSP90 levels were not found in this study(P>0.05). Conclusion] Expression levels of HSP27 and HSP70 can be induced by certain dosage of UVC irradiation. Such change in HSP90 was not found in this study.