RECK及MMP-14蛋白在神经母细胞瘤组织中的表达及与肿瘤转移关系的研究

目的 检测RECK蛋白及膜型基质金属蛋白酶MMP-14在神经母细胞瘤中的表达,探讨其在神经母细胞瘤的发生、发展、浸润和转移中的作用.方法 选择临床及病理资料齐全的存档神经母细胞瘤蜡块标本36例,另取节细胞神经瘤蜡块标本10例作对照.采用第二代通用型二步法监测系统(PV-6000)免疫组织化学方法 检测RECK及MMP-14蛋白的表达.结果 RECK蛋白在神经母细胞瘤组织中低表达(16.7%),并随肿瘤浸润深度的加深、远处转移的发生而降低(P=0.015<0.05).MMP-14在神经母细胞瘤组织中高表达(58.3%),并随肿瘤浸润深度的加深、远处转移的发生而增高(P=0.002<0.05).神经母细胞瘤组织中RECK与MMP-14蛋白的表达呈负相关(r=-0.418,P<0.05).结论 神经母细胞瘤组织中RECK低表达,而MMP-14高表达.RECK及MMP-14可能成为评估神经母细胞瘤发生浸润转移的重要指标.

Expression of reversion-inducing cysteine-rich protein with Kazal motifs and matrix metalloproteinase-14 in neu-roblastoma and researches on their roles in tumor metastasis YU Dan, DONG Qian, YANG Chuan-min,

Objective To detect the expression of RECK (reversion-inducing eysteine-rieh protein with Kazal motifs), MMP-14 (matrix metalloproteinase-14), as well as their correlations in neuroblastoma and to investigate their roles in metastasis and development of the tumor. Methods PV-6000 immunohisto-chemistry was used to measure the expression of RECK and MMP-14, in 36 samples of neuroblastoma tissue selected from paraffin wax-embodied specimens with complete clinicopathological data,and another 10 cases of ganglioneuroma tissue. Results The expression rate of RECK in neuroblastoma was low (16.7%), and re-duced with the increased invasive depth and long-distance metastasis(P=0.015<0.05). The expression rate of MMP-14 in neuroblastoma was high (58.3%),and elevated with the increased invasive depth and long-distance metastasis (P=0.002<0.05). The expression of RECK was negatively correlated with the expres-sion of MMP-14 (r=-0.418, P<0.05). Conclusions RECK is lowly expressed in neuroblastoma, but MMP-14 is highly expressed. RECK,MMP-14 may be served as the markers in the estimation of metastasis and dissemination of neuroblastoma.