怀菊花期次生代谢物含量及同功酶活性动态变化研究

目的：研究怀菊花期次生代谢产物的合成代谢规律及同工酶活性的动态变化。方法：测定怀菊花期总黄酮、绿原酸、花青苷的含量，以及苯丙氨酸解氨酶（PAL）、多酚氧化酶（PPO）、过氧化物酶（POD）的活性。结果与结论：怀菊花期各指标均呈先升高后下降的变化趋势，但高峰值出现的时间不同，总黄酮和绿原酸的高峰值出现在花开70％时，花青苷含量于花开50％时达到最高，PPO活性则在花开30％时最高，且2个栽培类型的变化趋势一致；PAL和POD的变化趋势因栽培类型的变化而有一定的差异，怀大白菊中PAL和POD活性在花开70％和30％时最高，而怀小白菊中则出现在花开50％。

Study on dynamic accumulation of secondary metabolites content and isoenzyme activity during blossoming stages in Chrysanthemum morifolium originating from Wenxian county

Objective: To study the anabolic rule of secondary metabolites and dynamic activity of isoenzyme in Chrysanthemum morifolium originating from Wenxian county during blossoming stages.Method: The flavonoid,chlorogenic acid and anthocyanin content as well as the PAL,PPO and POD activity were determined in C.morifolium originating from Wenxian county during blossoming stages.Result and Conclusion: The content of flavonoid and chlorogenic acid was the highest at 70% of full blossom,the anthocyanin at 50% and PPO activity at 30% with the same trend of two cultivars.Between the two cultivars,the trend of PAL and POD was different.The highest of "huaidabaiju" appeared at 70% and 30%,but that of "huaixiaobaiju" appeared at 50% and 50%.