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| 紫心甘薯多糖的抗氧化活性研究 | |
| 引用本文： | 高秋萍,阮红,毛童俊,刘森泉,余莹莹,张如意. 紫心甘薯多糖的抗氧化活性研究[J]. 营养学报, 2011, 33(1) |
| 作者姓名： | 高秋萍 阮红 毛童俊 刘森泉 余莹莹 张如意 |
| 作者单位： | 1. 浙江大学生命科学学院,杭州,310027 2. 浙江大学城市学院医学与生命科学学院,杭州,310015 3. 浙江大学医学院杭州,310058 |
| 基金项目： | 杭州市科技局重点实验室科技创新项目 |
| 摘 要： | 目的探讨紫心甘薯多糖(PPSP)的体内外抗氧化活性。方法采用OH.、DPPH.和还原力反应体系,检测紫心甘薯多糖的体外抗氧化活性,并与维生素C(VC)进行比较。建立链脲佐菌素(STZ)诱导糖尿病大鼠模型,测定并比较PPSP组与模型组的肝脏和胰腺内丙二醛(MDA)含量、还原型谷胱甘肽(GSH)和总抗氧化(T-AOC)活性。结果体外抗氧化实验表明,随着紫心甘薯多糖浓度增加,其还原力、OH.和DPPH.清除率均有所增加。当PPSP浓度为250μg/ml时,表现出较强的还原力,对OH.和DPPH.的清除率分别为92.2%和60.2%,其中对OH.清除率明显高于VC,对DPPH.清除率接近VC清除水平。按PPSP低、中、高剂量(分别为100、200和400 mg/kg.bw)灌胃,结果表明大鼠肝脏和胰腺组织内的MDA含量都有明显降低,GSH和T-AOC活性都有明显升高。肝脏切片结果显示中剂量多糖能有效减少糖尿病所致的肝脏细胞损伤。结论紫心甘薯多糖具有较强的体内外抗氧化活性。 |
| 关 键 词： | 紫心甘薯 多糖 抗氧化活性 |
STUDY ON THE ANTIOXIDANT ACTIVITY OF POLYSACCHAARIDE FROM PURPLE SWEET POTATO |
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| GAO Qiu-ping,RUAN Hong,MAO Tong-jun,LIU Sen-quan,YU Ying-ying,ZHANG Ru-yi. STUDY ON THE ANTIOXIDANT ACTIVITY OF POLYSACCHAARIDE FROM PURPLE SWEET POTATO[J]. Acta Nutrimenta Sinica, 2011, 33(1) | |
| Authors: | GAO Qiu-ping RUAN Hong MAO Tong-jun LIU Sen-quan YU Ying-ying ZHANG Ru-yi |
| Affiliation: | GAO Qiu-ping,RUAN Hong1,MAO Tong-jun2,LIU Sen-quan1,YU Ying-ying1,ZHANG Ru-yi1(College of Life Sciences,Zhejiang University,Hangzhou 310058,1School of Medicine and Life Science,Zhejiang University City College,Hangzhou 310015,2College of Medicine,China) |
| Abstract: | Objective To investigate the antioxidant activity of polysaccharide from purple sweet potato(PPSP) in vivo and in vitro.Method The reaction system of hydroxyl radical,DPPH free radical and reducing power was used to detect the antioxidant activity of PPSP in vitro and was compared with VC.The contents of MDA,the activities of GSH and T-AOC in liver and pancreas were respectively measured in the diabetes mellitus mouse model induced by streptozotocin(STZ).Results The in vitro experiment showed that the reduc... |
| Keywords: | purple sweet potato alkali-soluble polysaccharide antioxidation |
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