依贝沙坦对自发性高血压大鼠左心室肥厚和心肌纤维化的影响

目的探讨依贝沙坦对自发性高血压大鼠(SHR)左心室肥厚(LVH)和心肌纤维化的影响。方法18只16周龄SHR,随机分为依贝沙坦治疗组(SHR-I)和SHR空白对照组(SHR-C);另设同源的WKY大鼠8只为正常对照组。治疗组口服依贝沙坦50mg.kg-1.d-1给药8周后处死动物,取左心室心肌称重,计算左心室/体重比(LVW/BW),Masson三色法染色观察左心室心肌胶原变化,计算机图象分析测量心肌切片的胶原容积分数(CVF)和血管周围胶原面积(PVCA)。结果SHR空白对照组的收缩压(SBP),LVW/BW,CVF,PVCA均显著高于WKY对照组(P<0.01),与SHR空白对照组相比,依贝沙坦治疗组能有效降低SHR的SBP,改善左心室肥厚(P<0.01)并使左心室内膜及心肌小动脉周围的胶原减少(P<0.01)。结论依贝沙坦可有效降低SHR血压,部分逆转心肌纤维化和左心室肥厚。

The Effects of Irbesartan on Left Ventricular Hypertrophy and Myocardial Fibrosis in SHR

Objictive To explore the effects of Irbesartan on left ventricular hypertrophy and myocardial fibrosis in spontaneously hypertensive rats.Methods 18 SHR at 16 weeks of age were randomly divded into active group and control group,The rats in active group were treated with 50mg·kg~(-1)·d~(-1)Irbesartan.Anther 8 rats were recruited as healthy control.After 8 weeks,all the rats were killde,and all the left ventricle muscle weight(LVW)were measured to calculate the LVW/BW;the interstitial collagen volume fraction(CVF)and content of collagen on pathological tissue slices were assessed with computerized video processing.Results compared with WKY rats,the SBP,LVMI,collagen volume fraction(CVF) and perivascular circumferential collagen area(PVCA)were significantly reduced in treated SHRs(P<0.01),and substantially reduced in treated SHRs than that in untreated SHRs(P<0.01).Conclusion Irbesartan can effectively decrease blood pressure,at same time their myocardial fibrosis and left vertricle hypertrophy can also be reversed partially.