新型超顺磁性氧化铁在雪旺细胞移植后活体示踪中的应用研究

目的：研究一种新型超顺磁性氧化铁（Superparamagnetic iron oxide，SPIO）在雪旺细胞（SCs）标记及磁共振（MRI）活体示踪中的应用。方法： 以二巯基丁二酸修饰三氧化二铁（Fe2O3）配制成一种新型SPIO，用于标记SCs。实验组用SPIO标记SCs，对照组不用SPIO标记，每组各10个样本。对标记后的SCs行普鲁士蓝染色鉴定、MTT测试细胞相对数量。最后将标记后的SCs移植入大鼠脑内，MRI扫描活体示踪。结果： 普鲁士蓝染色发现该SPIO可高效率标记SCs，MTT测试表明实验组与对照组的雪旺细胞相对数量无统计学差异（P〉0．05），MRI检查发现脑内移植的SPIO标记SCs在T。相上呈明显的低信号。结论：通过二巯基丁二酸修饰Fe2O2获得了新型SPIO，该新型SPIO对细胞增殖及活力无明显影响，不需要转染剂仍可高效率标记SCs，MRI活体显影示踪效果良好。

Application research of novel Superparamagnetic iron oxide in vivo tracing of transplanted schwann cells

AIM： To investigate a novel Superparamagnetic iron oxide （SPIO） used for la- beling Schwann cells （SCs） and tracing in vivo by magnetic resonance. METHODS： The novel SPIO was prepared with Fe203 coated by a thin layer of dimercaptosuccinic acid, and then it was used for labeling SCs. The SCs of trial group were labeled by SPIO. The SCs of control group weren＇t labeled by SPIO. Every group had ten samples. The labeled SCs were identified by Prussian blue stain and counted by MTT. Lastly, the labeled SCs was transplanted into rat brain and scanned by MRI. RESULTS： Prussian blue stain indicated that SCs could be high efficiently labeled by the novel SPIO. MTT detection suggested the difference of viable cells relative number was not significant between trial group and control group （P〈0.05）. Hypo-intense changes of MRI T2 examination could be observed in the transplanted area of rat brain labeled by the new SPIO after transplantation in rats brain. CONCLUSION. The novel Superparamagnetic iron oxide had no significant effect on the proliferation and viability of SCs. SCs still could be labelled with the novel SPIO directly and high efficiently without the use of poly-L-ly-sine or other additional transfecting agents. The transplanted cells could be well imaged and traced in vivo.