茯苓多糖对Lewis肺癌小鼠自发肺转移的抑制作用及其机制研究

目的 研究茯苓多糖对Lewis肺癌小鼠自发肺转移的影响，并探讨其作用机制。方法 C57BL/6小鼠右腋皮下接种Lewis肺癌细胞，分为茯苓多糖高（0.50 mg/只）、低（0.33 mg/只）剂量组，顺铂组（顺铂0.04 mg/只），模型组（生理盐水），接种瘤细胞后第2天开始尾iv给药，隔天一次。观察小鼠一般状态，造模后第7天开始，隔天测量肿瘤体积。造模后21 d取肺，计数肺表面转移灶个数，HE染色检测肺微小转移灶个数，检测外周血白细胞数量及脾质量、脾指数。结果 茯苓多糖高、低剂量对实体瘤无明显抑制，茯苓多糖高剂量可减少肺表面转移灶个数，增加白细胞CD11bmRNA表达，不影响外周血白细胞数、脾质量及脾指数，一般状况较好；茯苓多糖低剂量对肺表面转移灶个数、外周血白细胞数、脾质量及脾指数无明显影响，可增加白细胞CD11b、CD18mRNA表达，一般状况较好。结论 茯苓多糖对Lewis肺癌小鼠实体瘤无明显抑制作用，但能够抑制其自发肺转移，对外周血白细胞数量、脾质量及脾指数无明显影响，可增加外周血白细胞CD11b、CD18mRNA表达，活化外周血白细胞可能是茯苓多糖抑制肿瘤转移的机制之一。

Inhibition of pachymaran on spontaneous lung metastasis in Lewis lung cancer of mice and its mechanism

Objective To investigate the effects and mechanism of pachymaran on spontaneous lung metastasis in Lewis lung cancer mice model. Methods C57BL/6 mice were sc injected with Lewis lung cells in the right axilla, and the mice were divided into four groups with ten mice each, such as model （physiological saline）, cisplatin （0.04 mg）, and pachymaran high- and low-dose （0.50 and 0.33 mg） groups, and from the day 2 of model establishment, the drug was given through tail vein every 2 d. The tumor volume was measured from 7 d to 21 d every two days. The number of metastasis on the surface of the lung was calculated after 21 d. The number of micrometastases in lung was calculated by HE staining. The numbers of peripheral blood leukocytes, spleen weight, and spleen index were also detected. Results The low- and high-dose pachymaran had no effect on the growth of the tumor. The high-dose pachymaran could reduce the number of lung surface metastases and increase the expression of CD11b mRNA in peripheral white blood cells. It had no effect on the number of the peripheral blood leukocyte, spleen weight, and spleen index. The low-dose pachymaran had no effect on the number of lung surface metastases, the number of the peripheral blood leukocyte, spleen weight, and spleen index. But it could increase the expression of CD11b mRNA and CD18 mRNA in peripheral white blood cells. Conclusion Pachymaran has no obvious inhibitory effect on Lewis lung cancer solid tumor in mice, but could inhibit the spontaneous lung metastasis. It has no significant effect on peripheral blood white cell count, spleen weight, and spleen index, but it may increase the expression of CD11b and CD18mRNA of peripheral white blood cells. It is suggested that the activation of peripheral white blood cells could be involved in the mechanism of pachyman inhibitory mechanism of tumor metastasis.