| 强脉冲光对小鼠皮肤胶原及PCNA表达的影响 |
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| 引用本文： | 欧阳泽辉,黄进华,康〓健,耿雪瑞,于小峰,鲁建云,陈〓静. 强脉冲光对小鼠皮肤胶原及PCNA表达的影响[J]. 南京医科大学学报(自然科学版), 2010, 0(9): 1258-1261 |
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| 作者姓名： | 欧阳泽辉 黄进华 康〓健 耿雪瑞 于小峰 鲁建云 陈〓静 |
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| 作者单位： | 中南大学湘雅第三医院皮肤科,湖南 长沙〓410013;中南大学湘雅第三医院皮肤科,湖南 长沙〓410013;中南大学湘雅第三医院皮肤科,湖南 长沙〓410013;中南大学湘雅第三医院皮肤科,湖南 长沙〓410013;中南大学湘雅第三医院皮肤科,湖南 长沙〓410013;中南大学湘雅第三医院皮肤科,湖南 长沙〓410013;中南大学湘雅第三医院皮肤科,湖南 长沙〓410013 |
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| 摘 要： | 目的:观察强脉冲光(intense pulsed light,IPL)对小鼠皮肤的胶原纤维及细胞增殖核抗原(proliferating cell nuclear antigen,PCNA)的影响,探讨强脉冲光嫩肤的分子生物学机制?方法:采用能量密度为32 J/cm2强脉冲光照射小鼠背部右侧皮肤,左侧为对照,分别于照射后1?7?14?28天切取皮肤样本,行HE染色,苦味酸-酸性品红染色(VG 染色) 观察皮肤组织及胶原变化,免疫组化检测皮肤PCNA的表达?结果:与对照部位比较,IPL照射对真皮厚度无明显改变(P < 0.05);照射后1?7天两侧皮肤胶原含量无明显改变(P > 0.05),14及28天后照射部位皮肤胶原明显增加 (P < 0.05);PCNA表达于照射后1?7天无明显改变(P > 0.05),14及28天显著增强(P < 0.05)?结论:强脉冲光可促进皮肤胶原含量增加,并可诱导PCNA表达增强?
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| 关 键 词： | 强脉冲光 胶原 PCNA |
| 收稿时间： | 2010-04-11 |
Effects of intense pulsed light on collagens and PCNA expression in mouse skin |
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| OUYANG Ze-hui,HUANG Jin-hu,KANG Jian,GENG Xue-rui,YU Xiao-feng,LU Jian-yun and CHEN Jing. Effects of intense pulsed light on collagens and PCNA expression in mouse skin[J]. Acta Universitatis Medicinalis Nanjing, 2010, 0(9): 1258-1261 |
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| Authors: | OUYANG Ze-hui HUANG Jin-hu KANG Jian GENG Xue-rui YU Xiao-feng LU Jian-yun CHEN Jing |
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| Affiliation: | Department of Dermatology,the Third Xiangya Hospital of Central South University,Changsha 410013,China;Department of Dermatology,the Third Xiangya Hospital of Central South University,Changsha 410013,China;Department of Dermatology,the Third Xiangya Hospital of Central South University,Changsha 410013,China;Department of Dermatology,the Third Xiangya Hospital of Central South University,Changsha 410013,China;Department of Dermatology,the Third Xiangya Hospital of Central South University,Changsha 410013,China;Department of Dermatology,the Third Xiangya Hospital of Central South University,Changsha 410013,China;Department of Dermatology,the Third Xiangya Hospital of Central South University,Changsha 410013,China |
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| Abstract: | Objective:To observe the effect of intense pulsed light (IPL) on collagens and PCNA expressions in mouse skin and elucidate the biological mechanisms of photorejuvenation. Methods:Mice were irradiated on their right sides of the back with IPL(32 J/cm2),and their left sides were selected as control areas. At day 1,7,14 and 28 after treatment,skin specimens were taken at treated and non-treated areas,respectively. Histological and collagens changes were observed by hematoxylin-eosin-staining and Van Gieson staining. In addition,the expression of PCNA was investigated by immunohistochemistry at day 28 after irradiation. Results:Compared with non-treated regions,dermal thickness of treated regions had no significant change at day 1,7,14 and 28(P > 0.05). Collagens staining were stronger in treated regions than in non-treated areas at day 14 and 28(P < 0.05). The expression of PCNA also increased at day 14 and 28 after irradiation in treated areas(P < 0.05). Conclusion:IPL can enhance the content of collagens and also can increase the expression of PCNA in mouse skin. |
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| Keywords: | intense pulsed light collagen PCNA |
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