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强脉冲光对小鼠皮肤胶原及PCNA表达的影响
引用本文:欧阳泽辉,黄进华,康〓健,耿雪瑞,于小峰,鲁建云,陈〓静.强脉冲光对小鼠皮肤胶原及PCNA表达的影响[J].南京医科大学学报,2010(9):1258-1261.
作者姓名:欧阳泽辉  黄进华  康〓健  耿雪瑞  于小峰  鲁建云  陈〓静
作者单位:中南大学湘雅第三医院皮肤科,湖南 长沙〓410013;中南大学湘雅第三医院皮肤科,湖南 长沙〓410013;中南大学湘雅第三医院皮肤科,湖南 长沙〓410013;中南大学湘雅第三医院皮肤科,湖南 长沙〓410013;中南大学湘雅第三医院皮肤科,湖南 长沙〓410013;中南大学湘雅第三医院皮肤科,湖南 长沙〓410013;中南大学湘雅第三医院皮肤科,湖南 长沙〓410013
摘    要:目的:观察强脉冲光(intense pulsed light,IPL)对小鼠皮肤的胶原纤维及细胞增殖核抗原(proliferating cell nuclear antigen,PCNA)的影响,探讨强脉冲光嫩肤的分子生物学机制?方法:采用能量密度为32 J/cm2强脉冲光照射小鼠背部右侧皮肤,左侧为对照,分别于照射后1?7?14?28天切取皮肤样本,行HE染色,苦味酸-酸性品红染色(VG 染色) 观察皮肤组织及胶原变化,免疫组化检测皮肤PCNA的表达?结果:与对照部位比较,IPL照射对真皮厚度无明显改变(P < 0.05);照射后1?7天两侧皮肤胶原含量无明显改变(P > 0.05),14及28天后照射部位皮肤胶原明显增加 (P < 0.05);PCNA表达于照射后1?7天无明显改变(P > 0.05),14及28天显著增强(P < 0.05)?结论:强脉冲光可促进皮肤胶原含量增加,并可诱导PCNA表达增强?

关 键 词:强脉冲光    胶原    PCNA
收稿时间:2010/4/11 0:00:00

Effects of intense pulsed light on collagens and PCNA expression in mouse skin
OUYANG Ze-hui,HUANG Jin-hu,KANG Jian,GENG Xue-rui,YU Xiao-feng,LU Jian-yun and CHEN Jing.Effects of intense pulsed light on collagens and PCNA expression in mouse skin[J].Acta Universitatis Medicinalis Nanjing,2010(9):1258-1261.
Authors:OUYANG Ze-hui  HUANG Jin-hu  KANG Jian  GENG Xue-rui  YU Xiao-feng  LU Jian-yun and CHEN Jing
Institution:Department of Dermatology,the Third Xiangya Hospital of Central South University,Changsha 410013,China;Department of Dermatology,the Third Xiangya Hospital of Central South University,Changsha 410013,China;Department of Dermatology,the Third Xiangya Hospital of Central South University,Changsha 410013,China;Department of Dermatology,the Third Xiangya Hospital of Central South University,Changsha 410013,China;Department of Dermatology,the Third Xiangya Hospital of Central South University,Changsha 410013,China;Department of Dermatology,the Third Xiangya Hospital of Central South University,Changsha 410013,China;Department of Dermatology,the Third Xiangya Hospital of Central South University,Changsha 410013,China
Abstract:Objective:To observe the effect of intense pulsed light (IPL) on collagens and PCNA expressions in mouse skin and elucidate the biological mechanisms of photorejuvenation. Methods:Mice were irradiated on their right sides of the back with IPL(32 J/cm2),and their left sides were selected as control areas. At day 1,7,14 and 28 after treatment,skin specimens were taken at treated and non-treated areas,respectively. Histological and collagens changes were observed by hematoxylin-eosin-staining and Van Gieson staining. In addition,the expression of PCNA was investigated by immunohistochemistry at day 28 after irradiation. Results:Compared with non-treated regions,dermal thickness of treated regions had no significant change at day 1,7,14 and 28(P > 0.05). Collagens staining were stronger in treated regions than in non-treated areas at day 14 and 28(P < 0.05). The expression of PCNA also increased at day 14 and 28 after irradiation in treated areas(P < 0.05). Conclusion:IPL can enhance the content of collagens and also can increase the expression of PCNA in mouse skin.
Keywords:intense pulsed light  collagen  PCNA
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