| Wistar大鼠急性肾脏缺血再灌注损伤动物模型的建立及意义 |
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| 引用本文： | 姜锡男,陈方敏,石家齐,严〓波,谷〓江,张〓亚,李登宝,沈〓俊,梁〓骏,任德帅. Wistar大鼠急性肾脏缺血再灌注损伤动物模型的建立及意义[J]. 南京医科大学学报(自然科学版), 2010, 0(8): 1108-1111 |
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| 作者姓名： | 姜锡男 陈方敏 石家齐 严〓波 谷〓江 张〓亚 李登宝 沈〓俊 梁〓骏 任德帅 |
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| 作者单位： | 贵阳医学院附属医院泌尿外科,贵州 贵阳〓550004;贵阳医学院附属医院泌尿外科,贵州 贵阳〓550004;贵阳医学院附属医院泌尿外科,贵州 贵阳〓550004;贵阳医学院附属医院泌尿外科,贵州 贵阳〓550004;贵阳医学院附属医院泌尿外科,贵州 贵阳〓550004;贵州省肿瘤医院妇瘤外科,贵州 贵阳〓550004;贵阳医学院附属医院泌尿外科,贵州 贵阳〓550004;贵阳医学院附属医院泌尿外科,贵州 贵阳〓550004;贵阳医学院附属医院泌尿外科,贵州 贵阳〓550004;贵阳医学院附属医院泌尿外科,贵州 贵阳〓550004 |
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| 基金项目： | 贵州省优秀科技教育人才省长基金专项基金项目(黔省专合字2006-59），贵阳医学院附属医院博士启动基金资助 |
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| 摘 要： | 目的:建立一种简单?安全?高成功率的大鼠急性肾脏缺血再灌注损伤动物模型?方法:应用微型动脉夹夹闭Wistar大鼠双侧肾动脉50 min后松开,于伤后第3?5?7天取肾,经一系列处理后进行HE染色及免疫组化染色,以观察在肾脏近曲小管上皮细胞中是否出现肾损伤分子-1(kidney injury molecule-1,Kim-1)抗原及该细胞形态学改变?结果:该法造模成功率达93.3%,实验组肾脏近曲小管上皮细胞Kim-1抗原表达率上升,同时该细胞有明显细胞形态学改变(P > 0.05)?结论: 应用微型动脉夹夹闭Wistar大鼠双侧肾动脉可制备急性肾脏缺血再灌注损伤模型,且成功率高?方法简单?
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| 关 键 词： | 急性肾脏缺血再灌注损伤 动物模型 |
| 收稿时间： | 2010-01-22 |
Model construction of renal acute ischemia-reperfusion injury in Wistar rats |
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| JIANG Xi-nan,CHEN Fang-min,SHI Jia-qi,YAN Bo,GU Jiang,ZHANG Y,LI Deng-bao,SHEN Jun,LIANG Jun and REN De-shuai. Model construction of renal acute ischemia-reperfusion injury in Wistar rats[J]. Acta Universitatis Medicinalis Nanjing, 2010, 0(8): 1108-1111 |
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| Authors: | JIANG Xi-nan CHEN Fang-min SHI Jia-qi YAN Bo GU Jiang ZHANG Y LI Deng-bao SHEN Jun LIANG Jun REN De-shuai |
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| Affiliation: | Department of Urology,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004;Department of Urology,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004;Department of Urology,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004;Department of Urology,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004;Department of Urology,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004;Department of Gynaecology and Cancer,Guizhou Cancer Hospital,Guiyang 550004,China;Department of Urology,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004;Department of Urology,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004;Department of Urology,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004;Department of Urology,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004 |
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| Abstract: | Objective:To construct a simple,safe,high successful rate model of renal acute ischemia-reperfusion injury in Wistar rats. Methods:Renal artery of Wistar rats was bilaterally clamped with a micro-artery clamp for 50 minutes to make the animal model of renal acute ischemia-reperfusion injury. Then these kidneys were removed at the 3rd,5th,7th day after injury. Immunohistochemistry staining was used to show the distribution and expression of Kim-1 antigen,and the changes of cytomorphology were observed by HE staining. Results:The successful rate of model construction reached to 85% in the experiment. The expression of Kim-1 antigen increaced. Meanwhile,changes of cytomorphology took place. Conclusion:Stable model of renal acute ischemia-reperfusion injury of Wistar rats can be constructed by applying micro-artery clamp to incarcerate bilateral renal arteries,and its successful rate is very high. |
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| Keywords: | renal acute ischemia-reperfusion injury animal model |
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