大鼠单肺原位移植模型的建立及改良

目的:改进大鼠原位单肺移植模型建立的方法,建立稳定的动物模型?方法:将46只健康SD雄性大鼠随机配对,采用三袖套法进行肺动?静脉及支气管的吻合,建立大鼠肺移植模型?结果:手术成功20例,失败3例,其中肺静脉撕裂1例,吻合肺动脉失败1例,术后出血失败1例?供肺获取时间为(11.6±2.1)min,供肺套管时间为(13.6±2.4)min,动静脉和支气管套管吻合时间为(17.7±3.5)min?血气?胸片及病理检查证实成功建立大鼠单肺原位移植模型?结论:改良的建立大鼠单肺原位移植模型的方法能够更好的满足肺移植研究的需要?

Establishment and improvement of rat unilateral orthotopic lung transplantation model

Objective:To improve the technique of rat unilateral orthotopic lung transplantation and establish a stable animal model. Methods:Forty-six male Sprague-Dawley rats were randomly divided into 23 pairs for donors and recipients. Cuff techniques were introduced in anastomosis of bronchus,pulmonary artery and pulmonary vein in the operation. Results:In the 23 transplantations,the operation was successfully carried out in 20 cases and failed in 3 cases. The reasons of failure were as follows:disruption of pulmonary vein,disruption of pulmonary artery and bleeding after operation. Time for lung harvesting,cuff techniques and anastomosis was(11.6±2.1)min,(13.6±2.4)min and(17.7±3.5)min respectively. The graft function was monitored by arterial blood gas analysis,thoracic X ray and pathological examination. Recipients after transplantation showed excellent graft function. Conclusion:The cuff techniques mentioned in this study would be more suitable for establishment of rat unilateral orthotopic lung transplantation model.