survivin?cyclin D1 在结直肠癌组织中的表达及其临床意义

目的:探讨survivin?cyclin D1 在结直肠癌中的表达及其与临床病理特征的关系?方法:43例结直肠癌组织标本,应用实时荧光定量PCR 对survivin?cyclin D1基因在结直肠癌组织及癌旁正常组织中的mRNA 水平进行定量检测,同时应用蛋白免疫印记(Western blot)检测其在结直肠癌组织及癌旁正常组织中蛋白水平的表达?结果:结直肠癌组织survivin?cyclin D1 mRNA 和蛋白水平的表达均较癌旁正常组织表达显著上调,其差异有统计学意义(P﹤0.05);survivin?cyclin D1 mRNA 和蛋白水平的表达与患者性别?年龄?肿瘤部位?肿块大小?浸润深度无显著相关性(P﹥0.05),而与TNM 分期?淋巴结转移有显著相关(P﹤0.05)?结论:提示survivin?cyclin D1基因在结直肠癌的发生?发展过程及预后中可能具有重要作用,有望成为新的肿瘤标志物或预后因子?

Expressions of survivin and cyclin D1 and their clinical significance in human colorectal carcinoma

Objective:To investigate the expression of survivin,cyclin D1 in human colorectal carcinoma and their relationship with clinicopathological features. Methods:The mRNA expression of survivin and cyclin D1 was determined by real-time quantitative PCR in 43 colorectal carcinoma tissues and para-carcinoma normal tissues. Subsequently,Western blot was used to quantitatively analyze the protein levels in colorectal carcinoma tissues and para-carcinoma normal tissues,respectively. Results:Real-time PCR and Western blot analysis revealed that survivin,cyclin D1 expression were also higher in colorectal carcinoma tissues than those in the paraneoplastic colorectal tissues(P < 0.01). There were no significant correlation between the copies of survivin,cyclin D1 and gender,age,tumor location,tumor size and depth of invasion(P > 0.05),but related to TNM stage and lymph node metastases,significantly(P < 0.05). Conclusion:Based on these findings,overexpression of survivin and cyclin D1 in the colorectal carcinoma suggested they may be participating in the genesis and development of colorectal carcinoma. Survivin and cyclin D1 could be useful tumor markers and prognostic factors for colorectal carcinoma.