| UPLC同时测定黄芩中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、千层纸素A |
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| 引用本文： | 刘金欣,孟繁蕴,张胜海,卢恒,周骁腾,侯静怡,李耿.UPLC同时测定黄芩中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、千层纸素A[J].中草药,2014,45(10):1477-1480. |
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| 作者姓名： | 刘金欣 孟繁蕴 张胜海 卢恒 周骁腾 侯静怡 李耿 |
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| 作者单位： | 北京师范大学 中药资源保护与利用北京市重点实验室, 北京 100875;北京师范大学 资源学院资源生态与中药资源研究所, 北京 100088;北京师范大学 中药资源保护与利用北京市重点实验室, 北京 100875;北京师范大学 资源学院资源生态与中药资源研究所, 北京 100088;北京中医药大学, 北京 100102;北京师范大学 中药资源保护与利用北京市重点实验室, 北京 100875;北京师范大学 资源学院资源生态与中药资源研究所, 北京 100088;北京师范大学 中药资源保护与利用北京市重点实验室, 北京 100875;北京师范大学 资源学院资源生态与中药资源研究所, 北京 100088;北京师范大学 中药资源保护与利用北京市重点实验室, 北京 100875;北京师范大学 资源学院资源生态与中药资源研究所, 北京 100088;北京师范大学 中药资源保护与利用北京市重点实验室, 北京 100875;中日友好医院 全国中西医结合心血管病中心, 北京 100029 |
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| 基金项目： | 国家自然科学基金资助项目（81072999） |
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| 摘 要： | 目的 建立用超高效液相色谱（UPLC）同时测定黄芩中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、千层纸素A 5种成分的方法。方法 采用UPLC色谱系统，BEH C18色谱柱（50 mm×2.1 mm，1.7 μm）；流动相为乙腈-0.1%甲酸，梯度洗脱，体积流量为0.6 mL/min；进样量为2 μL；检测波长为280 nm。结果 本方法可在15 min内完成1次色谱分析，黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、千层纸素A色谱峰之间有良好的分离度，5种成分的浓度和各自峰面积之间有着良好的线性关系，精密度、重复性及加样回收率的RSD均小于3.0%。结论 本方法快捷、准确，重复性好，能同时测定黄芩中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、千层纸素A 5种成分的量。
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| 关 键 词： | 黄芩 黄芩苷 黄芩素 汉黄芩苷 汉黄芩素 千层纸素A 超高效液相色谱 |
| 收稿时间： | 1/2/2014 12:00:00 AM |
Simultaneous determination of baicalin, wogonoside, baicalein, wogonin, and oroxylin-A in Scutellaria baicalensis by UPLC |
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| LIU Jin-xin,MENG Fan-yun,ZHANG Sheng-hai,LU Heng,ZHOU Xiao-teng,HOU Jing-yi and LI Geng.Simultaneous determination of baicalin, wogonoside, baicalein, wogonin, and oroxylin-A in Scutellaria baicalensis by UPLC[J].Chinese Traditional and Herbal Drugs,2014,45(10):1477-1480. |
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| Authors: | LIU Jin-xin MENG Fan-yun ZHANG Sheng-hai LU Heng ZHOU Xiao-teng HOU Jing-yi and LI Geng |
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| Institution: | Beijing Key Laboratory of Protection and Application of Chinese Medicinal Resources, Beijing Normal University, Beijing 100875, China;Institute of Natural Medicine and Chinese Medicine Resources, College of Resource Science & Technology, Beijing Normal University, Beijing 100088, China;Beijing Key Laboratory of Protection and Application of Chinese Medicinal Resources, Beijing Normal University, Beijing 100875, China;Institute of Natural Medicine and Chinese Medicine Resources, College of Resource Science & Technology, Beijing Normal University, Beijing 100088, China;Beijing University of Chinese Medicine, Beijing 100102, China;Beijing Key Laboratory of Protection and Application of Chinese Medicinal Resources, Beijing Normal University, Beijing 100875, China;Institute of Natural Medicine and Chinese Medicine Resources, College of Resource Science & Technology, Beijing Normal University, Beijing 100088, China;Beijing Key Laboratory of Protection and Application of Chinese Medicinal Resources, Beijing Normal University, Beijing 100875, China;Institute of Natural Medicine and Chinese Medicine Resources, College of Resource Science & Technology, Beijing Normal University, Beijing 100088, China;Beijing Key Laboratory of Protection and Application of Chinese Medicinal Resources, Beijing Normal University, Beijing 100875, China;Institute of Natural Medicine and Chinese Medicine Resources, College of Resource Science & Technology, Beijing Normal University, Beijing 100088, China;Beijing Key Laboratory of Protection and Application of Chinese Medicinal Resources, Beijing Normal University, Beijing 100875, China;National Integrative Medicine Center for Cardiovascular Disease, China-Japan Friendship Hospital, Beijing 100029, China |
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| Abstract: | |
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| Keywords: | Scutellaria baicalensis Georgi baicalin wogonoside baicalein wogonin oroxylin-A UPLC |
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