X射线交错互补修复基因1多态性和EB病毒感染对鼻咽癌细胞基本特性的影响

目的　探讨X射线交错互补修复基因1（X-ray repair cross-complementing gene 1，XRCC1）多态性与EB病毒感染对鼻咽癌细胞基本特性的影响。方法　将鼻咽癌细胞株分组，使之分别表达不同的XRCC1基因型，转染EB病毒，比较转染前后不同基因型鼻咽癌细胞的基本特性，并检测XRCC1蛋白的表达水平。结果　XRCC1不同基因型的鼻咽癌细胞之间基本特性无明显差异，XRCC1蛋白水平无差异。EB病毒感染后，各组鼻咽癌细胞生长加快、增殖能力变强、迁移速度加快、凋亡率降低，XRCC1蛋白水平无明显改变。EB病毒感染后，194位点突变型的鼻咽癌细胞与280、399位点突变型细胞相比，细胞增殖和迁移能力改变程度较小，差异有统计学意义。其他基因型细胞基本特性及XRCC1蛋白水平无差异。结论　EB病毒感染可增加鼻咽癌易感性，EB病毒感染后XRCC1 194位点的Trp突变型基因可能对鼻咽癌有保护作用。

Study on XRCC1 gene polymorphisms and EBV infection in nasopharyngeal carcinoma and underlying mechanism

OBJECTIVE To investigate the relationship between genetic polymorphim of X-ray repair cross complementing gene 1(XRCC1) and EBV infection in NPC cell.METHODS NPC CNE cell strain was divided into different groups depending on its XRCC1 genetic polymorphism,and EBV was transferred into different groups.Characteristics of cells and XRCC1 protein were detected among different groups before and after EBV transferring.RESULTS There was no difference in cell characteristics and XRCC1 protein level among different genotypes in NPC CNE cells strain.After EBV transferring,there was an increasing in cell malignancy in NPC cells,including control group,wild type group,and 194,280,399 mutant genotype groups,and the differences were statistically significant(P>0.05).There was no difference in XRCC1 protein level before and after EBV transferring.Compared with other groups,the increasing in cell malignancy was less in the 194 mutant genotype group before and after EBV transferring,especially in cell migration and proliferation.There were no difference in the increasing of cell malignancy and XRCC1 protein level among control group,wild type and 280,399 mutant genotypes of NPC CNE cells before and after EBV transferring.CONCLUSION The results suggest that EBV transferring can raise the carcinogenicity of NPC cell.After EBV transferring,the 194 mutant genotype is associated with a reduced carcinogenicity of NPC cells compared with other genotypes.