IL-12刺激肥大细胞IL-13分泌与ERK信号转导通路的关系

目的: 检测IL-12对肥大细胞介质分泌的影响并探讨其可能的信号转导通路。方法：小鼠肥大细胞P815培养后，用不同浓度IL-12激发后收集细胞和上清，上清用酶联免疫吸附试验（ELISA）检测组胺、IL-6和IL-13的分泌量，P815细胞用细胞激发信号ELISA（CASE）方法检测信号转导通路蛋白ERK和P38磷酸化情况。 结果： 不同浓度IL-12(0、1.0、10.0和100.0 μg•L^-1)激发后，P815细胞IL-13分泌量较基础分泌量均增加，并且随IL-12浓度增加而增加;而P815细胞IL-6和组胺释放量与基础分泌量比较差异均无显著性。ERK信号转导通路抑制剂PD98059、U0126预处理的经不同浓度IL-12激发P815细胞后，细胞内ERK磷酸化百分比较未加抑制剂组均显著降低（P<0.05）；且P815细胞IL-13分泌量较未加抑制剂组亦显著降低（P<0.05）。P38信号转导通路抑制剂SB203580预处理的不同浓度IL-12激发P815细胞后，细胞内P38磷酸化百分比与未加抑制剂组比较差异均无显著性；且P815细胞IL-13分泌量与未加抑制剂组比较差异均无显著性。结论：IL-12刺激肥大细胞P815分泌IL-13可能是通过激活ERK信号转导通路实现的。

Relationship between IL-13 release of mase cells induced by IL-12 and ERK signaling pathway

Objective To investigate the effect of IL-12 on mediator release from mast cells and the potential signal transduction pathways correspondingly.Methods P815 cells were challenged with various concentrations of IL-12.The supernatants were collected and analyzed by enzyme-linked immunosorbent assay(ELISA) to detect the quantity of released IL-13,IL-6 and histamine.The cells were treated and analyzed by cellular activation of signal ELISA(CASE) to detect phosphorylation of ERK and P38.Results After incubated with different doses of IL-12(0,1.0,10.0 and 100.0 μg·L-1),the IL-13 release of P815 cells increased with the concentration of IL-12 compared with medium alone control (P<0.05);but there were no significant differences of IL-6 release and histamine release compared with mediun alone control.After P815 cells were incubated with PD98059 and U0126 prior to IL-12,the percentage of intracellular phosphorylation of ERK and IL-13 release decreased significantly compared with groups without inhibitor (P<0.05) .After P815 cells were incubated with SB203580 prior to IL-12,the percentage of intracellular phosphorylation of P38 and IL-13 release didn’t change compared with groups without inhibitor.Conclusion IL-12 induced IL-13 release from P815 cells is likely through activation of ERK signalling pathway.