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中药材和饮片的高效液相色谱指纹图谱鉴别
引用本文:罗国安 梁琼麟 王义明 胡坪 姜志宏. 中药材和饮片的高效液相色谱指纹图谱鉴别[J]. 世界科学技术, 2004, 6(5): 11-16
作者姓名:罗国安 梁琼麟 王义明 胡坪 姜志宏
作者单位:[1]清华大学药物研究所北京100084 [2]华东理工大学化学与制药学院上海200237 [3]香港浸会大学中医药学院香港
基金项目:科技部"十五"攻关项目(2002BA906A29-3)中药与天然药物有效成分检测技术和计量基标准研究,负责人:罗国安基础性科技专项(2002DEA20021-329)中药与天然药物有效成分指纹图谱检测技术研究,负责人:罗国安和国家973项目(G1999054404)中药方剂关键科学问题基础研究
摘    要:目的:提出中药材和饮片的高效液相色谱指纹图谱(APLU)鉴别的原则和方法。方法:经过HPLC指纹图谱获取、验证和鉴别三个步骤,得出供鉴别用的对照指纹图谱,确定指标成分峰、特征峰的相对保留时间及范围,用于中药材和饮片的鉴别。结论:以丹参药材为例,所提出的原则与方法可以作为药材和饮片高效液相色谱指纹图谱鉴别用途。

关 键 词:鉴别 饮片 HPLC指纹图谱 高效液相色谱 中药材 指标成分 对照 原则 步骤 目的
修稿时间:2004-03-21

Identification of Chinese Medicinal Materials and Their Prepared Slices by HPLC Fingerprint
Luo Guoan,Liang Qionglin and Wang Yiming. Identification of Chinese Medicinal Materials and Their Prepared Slices by HPLC Fingerprint[J]. World Science and Technology(Modernization of Traditional Chinese Medicine), 2004, 6(5): 11-16
Authors:Luo Guoan  Liang Qionglin  Wang Yiming
Abstract:Objective: To formulate the principles and method for the identification of Chinese medicinal materials and their prepared slices by HPLC fingerprint. Method: To obtain the control fingerprints of the medicinal materials and their prepared slices to be used for identification and define their marker peaks, characteristic peaks and relative retention time and range for the identification of the medicinal materials and their prepared slices via the three steps of acquisition, verification and identification of HPLC fingerprint. Conclusion: Taking Radix Salviae Miltiorrhizae as an example, the principles and method proposed in this article can be used for the identification of Chinese medicinal materials and their prepared slices by HPLC fingerprint.
Keywords:Chinese medicinal materials and their prepared slices   identification   fingerprint   HPLC
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