不同检验方法在诊断女性生殖道沙眼衣原体感染的局限性

目的:研究不同方法对宫颈拭子样本沙眼衣原体的检测价值。方法:采集392例20~50岁无感染症状女性宫颈分泌物,分别进行培养、核酸扩增及两种免疫层析法(法国LAB、美国QUICK),进行沙眼衣原体(Chlamydia Trachomatis,CT)检测,培养阳性或其它任意检验结果两种或两种以上阳性,定为真阳性,称为"扩大的金标准"。结果:392例受检查者,按"扩大的金标准"CT感染者共46例,发病率为11.73%(46/392),培养、PCR、LAB、QUICK敏感性分别为58.70%、91·30%、95.65%、54.37%。特异性分别为100%、98.84%、63.29%、96.80%。阳性预测值分别为58.70%、91.30%、25.73%、96·15%。阴性预测值分别94.79%、98.84%、99.10%、94.10%。结论:应用单一实验诊断CT感染具有很大的局限性。

The evaluation of different testing methods for Chlamydia Tachomatis screening

Objective:To evaluate the value of detective screening of Chlamydia Trachomatis(CT) from cervical swabs by different methods.Method:Cervical swabs were collected from 392 asymptomtic cases and the CT was detect by cell culture,polymerase chain reaction(PCR) and two rapid immunological kits(French LAB and America quick).If cell culture is positive or other two or more methods are positive,defining as true positive or "expanding gold standard".Results:According to the "expanding gold standard",there were 46 cases diagnosed with CT infection.The chlamydia positivity was 11.73(46/392).The sensitivity of cell culture,PCR,French LAB and America Quick was 58.70,91.30,95.65 and 54.37 respectively.The specificities were 100,98.84,63.29 and 96.80 respectively.The positive predicting value of cell culture,PCR,French LAB and America Quick was 58.70,91.30,25.73 and 95.15.The negative predicting value was 94.79,98.84,99.10 and 94.10 respectively.Conclusion:Clinical diagnosis of female reproductive CT infection has some limitation by only one method.