乙型肝炎病毒的垂直传播

本文收集西安市13名中止妊娠妇女的胎儿,胎龄在20~32周。乙型肝炎表面抗原(HBsAg)阳性的妇女9名,阴性4名。分别提取胎儿白细胞及肝脏等脏器的DNA。由DNA杂交技术检测发现1名HBsAg阳性妇女的胎儿白细胞与肝脏乙型肝炎病毒(HBV) DNA阳性,其他脏器均阴性。结果证明HBV可以垂直传播。

Transplacental Transmission of Hepatitis B Virus

Thirteen fetuses from terminated pregnancies were collected. The age of gestation ranged from 20 to 32 weeks. Nine mothers of these fetuses were positive for HBsAg, 5 of them were also positive for HBeAg, HBV DNA. Four mothers were negative for HBsAg. DNA was extracted from fetal leukocytes, liver, heart, spleen, kidney, etc. HBV DNA was detected by dot hybridization. The results showed that HBV DNA was positive in leukocytes and liver of a fetus whose mother was positive for HBsAg, HBeAg. The liver HBV DNA of the fetus was about 23.1 kb by Southern blot. When digested by Hind II, it was about 3.2 kb 13.4 kb and 23.1 kb. So our data might serve as an evidence of transplacental transmission of HBV infection. Transplacental transmission seemed to be related to serum HBsAg, HBeAg, HBV DNA and leukocyte HBV DNA of mother.