趋化因子受体CXCR-4在卵巢癌增殖及转移中的作用

目的观察CAOV-3和HO8910卵巢癌细胞株趋化因子受体(CXCR-4)表达及对其增殖、迁移的作用。方法流式细胞术分析CAOV-3及HO8910细胞黏附分子表达;反转录-聚合酶链反应(RT-PCR)检测CXCR-4转录,观察不同浓度基质蛋白衍生因子(SDF)-1α对CAOV-3和HO8910细胞增殖、迁移及其表面黏附分子表达的影响,并采用激光共聚焦显微镜观察经SDF-1α作用后,CAOV-3细胞表面黏附分子分布变化与其移行能力变化的相关性。结果流式细胞术检测提示二株细胞均表达CD44(98.8%和97.7%)、CD29分子(67.8%和73.5%)和CD49d(45.8%vs37.2%),但不表达CD62e(4.2%vs3.2%);CAOV-3细胞尚表达CXCR-4分子,SDF-1α上调CAOV-3细胞CD29分子(67.8%vs93.2%)的表达,尤其是可显著上调CD49e的表达(25.8%vs82.2%,P<0.05),并可促进其细胞增殖和移行,CAOV-3细胞移行与其细胞表面黏附分子重分布有关。结论SDF-1α可促进CAOV-3细胞增殖和移行,上调CD49e和CD29的表达和重分布,在肿瘤的转移中具有重要作用。

Effects of chemokine receptor CXCR-4 on proliferation and migration of ovarian cancer cells in vitro

Objective To investigate the expression chemokine receptor CXCR-4 on CAOV-3 and HO8910 ovarian cancer cells and its role on proliferation and migration of ovarian cancer cells in vitro.Methods Expression of adhesion molecules and CXCR-4 on CAOV-3 and HO8910 ovarian cancer cell lines was determined by flow cytometry(FCM).RT-PCR assay was employed to detect the expression of CXCR-4 on both CAOV-3 and HO8910 cells.The effects of SDF-1α on proliferation,migration and expression of adhexion molecules on those cells were also investigated by MTT,FCM and transwell culture system.The distribution of adhesion molecule on CAOV-3 cells was observed through confocal microscope after the cells stimulated with SDF-1α at a concentration of 100 μg/L.Results Expression of CD44(98.8% vs 97.7%),CD29(67.8% vs 73.5%) and CD49d(45.8% vs 37.2%) were detected on both ovarian cancer cell lines,but there was almost no expression of CD62e found on those cells.FCM analysis also indicated that CAOV-3 expression of CXCR-4 and SDF-1α stimulate proliferation and migration of CAOV-3 cells.The adhesion molecules on CAOV-3 was up-regulated in the presence of SDF-1α,especially the expression of CD29(67.8% vs 93.2%,P<0.01) and CD49e(25.8% vs 82.2%,P<0.01).The confocal laser scanning microscopy confirmed that cell migration could trigger the establishment of polarized morphology of CAOV-3 cells and the redistribution of CD29 and CD49e in presence of SDF-1α.Conclusion SDF-1α,which promotes proliferation and migration of ovarian cancer cell by redistribution of membrane adhesion molecules in vitro,may have greatly implication on ovarian cancer metastasis.