子宫内膜癌中PTEN突变研究

目的:研究抑癌基因PTEN在子宫内膜癌中的表达,探讨其临床意义。方法:采用免疫组化法,对正常子宫内膜、子宫内膜增生过长、子宫内膜不典型增生各20例,子宫内膜癌50例及子宫内膜癌旁组织50例中PTEN的表达进行检测。结果:PTEN在正常子宫内膜、子宫内膜增生过长、子宫内膜不典型增生、子宫内膜癌旁组织及子宫内膜癌中表达缺失率逐渐上升,P<0.05,差异有显著性。表达缺失率与组织学类型、组织学分级有关,P<0.05,与子宫内膜腺癌肌层浸润深度、临床分期、淋巴结转移无关,P>0.05。结论:PTEN表达缺失是早期诊断子宫内膜腺癌较好的生物标志,参与子宫内膜癌的发生、发展。

Study of PTEN mutation in endometrial cancer

Objective:To study the expression and its clinical significance of PTEN in the pathogensis of endometrial carcinoma.Methods:A specific and sensitive immunohistochemical S-P assay was used to detect the expression of PTEN in paraffin-embedded sections of 20 cases of normal endometrium,endometrial hyperplasia and atypical hyperplasia of endometrial,50 the tissue near the endometrial carcinoma and endometrial carcinoma.Results:The loss expression rates of PTEN in normal proliferative endometrium,endometrial hyperplasia,atypical hyperplasia of endometrial,the tissue near the endometrial carcinoma,and endometrial carcinoma were gradually increased.The difference was significant.In endometrial carcinoma the loss of PTEN expression was significantly related to the tissue type(P<0.05)and the cell grade(P<0.05),but not related to the depth of myometrium invasion and the clinical stage(P>0.05).Conclusion:The loss of PTEN may be used as a diagnostic biomarker for the earliest endometrial carcinoma,PTEN may play some roles in the carcinogenesis and development of endometrial carcinoma.