| 新生大鼠海马神经元的改良培养 |
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| 引用本文： | 高小青,杜杰,邓莉,郭侃,杨朝鲜,王特为.新生大鼠海马神经元的改良培养[J].泸州医学院学报,2011,34(3):228-230. |
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| 作者姓名： | 高小青 杜杰 邓莉 郭侃 杨朝鲜 王特为 |
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| 作者单位： | 1. 泸州医学院医学基础研究中心,四川泸州,646000
2. 泸州医学院教务处,四川泸州,646000 |
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| 基金项目： | 四川省教育厅项目(2006A155); 四川省科技厅项目(2006J13-005-1) |
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| 摘 要： | 探讨体外培养高纯度神经元的方法。方法：体外培养新生大鼠海马神经干细胞，再使神经干细胞在无血清N2添加剂培养基条件下向神经元方向分化。结果：从新生大鼠海马组织培养出的神经干细胞，表达巢蛋白，神经干细胞的分化细胞绝大部分表达MAP2，极少数几个细胞表达GFAP。结论：通过本实验方法可获得高纯度的神经元，为后续实验奠定基础。
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| 关 键 词： | 细胞培养 神经干细胞 神经元 大鼠 |
MPROVED CULTURE OF HIPPOCAMAL NEURONS OF NEONATAL RAT |
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| Gao Xiaoqing,et al.MPROVED CULTURE OF HIPPOCAMAL NEURONS OF NEONATAL RAT[J].Journal of Luzhou Medical College,2011,34(3):228-230. |
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| Authors: | Gao Xiaoqing |
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| Institution: | Gao Xiaoqing,et al Department of Anatomy and Neurobiology,Luzhou Medical College |
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| Abstract: | Objective: To investigate the method of obtaining high content of neuron in vitro.Methods: Hippocamal neural stem cells(NSCs) of neonatal rat were cultured in vitro,and then differentiated to neuron direction in serum-free medium containing N2 supplement.Results: The NSCs from hippocampus of neonatal rat expressed nestin,and most differentiated cells of NSCs expressed MAP2,sporadically several differentiated cells expressed GFAP.Conclusion: Highly purified neuron can be obtained by the method adopted by aut... |
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| Keywords: | Cultivation Neural stem cells Neuron Rat |
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