UPLC-MS/MS法检测止咳平喘类中药制剂中非法添加的茶碱

目的：建立止咳平喘类中药制剂中非法添加茶碱的检测方法.方法：采用超高效液相色谱-串联四极杆质谱,多反应监测（MRM）模式进行定性和定量检测.结果：茶碱的线性范围在1.0～200.0g·L-1之间,检出限为0.15 g·L-1,平均回收率为98.3%,RSD=2.2%（n=6）.样品筛查结果发现一批样品添加了茶碱.结论：本方法选择性强、灵敏度高、处理方法简单,测定快速,可用于止咳平喘类中药制剂中非法添加茶碱的测定.

Determination the Theophylline of Illegal Mixture in Traditional Chinese Medicines Preparation by UPLC-MS/MS

Objective： To establish a specific method for determination of the theophy]line in traditional chinese medicines prepa- ration. Method： Qualitative and quantitative analysis were performed by UPLC-MS/MS and multi-reactions monitoring（MRM） technology. Result： The standard curves of the theophylline showed good linearity over the concentration range of 1.0-200. 0 g. L- 1. The detection limits of the theophylline was 0.15 g·L-1 ,and the average recovery was 98. 3% ,RSD =2. 2% （n =6）. 12 samples were tested, theophylline was founded in one sample. Conclusion： The method was specific, sensitive, operate easily and quickly, and could be used to determination the theophylline of Illegal Mixture in traditional Chinese medicines preparation.