地黄中寡糖的提取分离工艺研究

目的研究地黄(Rehmannia glutinosa)中寡糖的提取分离工艺。方法建立水苏糖的HPLC测定法；以水苏糖含量为指标，采用正交试验优化地黄水提取物的提取工艺，对水提物进一步用离子交换树脂进一步纯化，再进行活性炭柱层析，以不同浓度梯度乙醇洗脱，分得寡糖的不同部位。结果地黄水提取的最佳工艺为A283C2，即溶剂(水)体积lO倍，提取3次，每次提取1h。水提物经活性炭柱层析得到4个部位，部位Ⅰ～Ⅳ，其中部位Ⅱ及部位Ⅲ中水苏糖的含量分别为20．99％及60．5l％。结论地黄水提取的较佳工艺条件为A283C2，活性炭柱层析可以较好地将地黄中寡糖进行分离，其中水苏糖的HPLC测定法简便、稳定、可靠。

Extraction and Separation Process of Oligosacchrides from Rehmannia Glutinosa

Aim To study extraction and separation process of oligosaccharides from Rehmannia glutinosa and HPLC determination of stachyose.Mehtods Orthogonal test was used to inspect the factors such as water volume,times and time of extraction by boiled water method from R.glutinosa.Oligosacchrides of water extract from R.glutinosa were further separated by charcoal column chromatography and the content of stachyose,the main component of water extract from R.glutinosa was determined by HPLC.Results According to the yields of water extract the influence factors were extraction time (B)> water volume (A)> time for extraction (C) in turn.The optimal extraction condition was found to be A 2B 3C 2,that is,with 10 volume of water for 1 hour,3 times.Water extract was further separated by charcoal column chromatography into four groups and identified by HPLC.It was found that the analysis of stachyose in water extract by HPLC was a stable and reliable method to determine the content of stachyose in the extract.Conclusion Oligosaccharides in R.glutinosa could be extracted at a fuller extent by boiled water method according to best procession of A 2B 3C 2,that was with 10 volume of water for 1 hour,3 times.Determination of stachyose by HPLC was stable and reliable.