治伤巴布剂的镇痛作用及对大鼠背根神经节辣椒受体表达的影响

目的：探讨治伤巴布剂对福尔马林足底炎性疼痛大鼠模型背根神经节中辣椒素受体水平的影响,研究治伤巴布剂对大鼠炎性疼痛是否有镇痛作用。方法：SD大鼠36只,随机分为空白对照组（n=12）、模型组（n=12）及治疗组（n=12）;空白对照组大鼠不予任何处理,模型组左后足底注射0.1 mL5%福尔马林,治疗组大鼠造模前1 d于左后足外敷治伤巴布剂,然后左后足底注射0.1 mL5%福尔马林,再一直外敷治伤巴布剂,观察造模后大鼠疼痛行为学反应,并以Dubuisson评分方法进行记录比较。各组大鼠在指定时间点取出L3-6节段背根神经节,运用实时荧光定量（RT-PCR）、蛋白质印迹（Western blot）检测辣椒素受体在大鼠背根神经节中的表达水平。结果：模型组及治疗组呈典型的双相伤害性行为反应,但治疗组大鼠疼痛反应积分低于模型组,差异有统计学意义（P〈0.05﹚;实时荧光定量检查结果显示模型组辣椒素受体mRNA相对表达量大于治疗组,治疗组辣椒素受体mRNA相对表达量大于空白对照组,3组比较,差异有统计学意义（P〈0.05﹚,3组组内3个时相点的相对表达量差异均无统计学意义（P〉0.05）;蛋白质印迹法灰度扫描显示模型组辣椒素受体相对表达量大于治疗组,治疗组辣椒素受体相对表达量大于空白对照组,3组比较,差异有统计学意义（P〈0.05﹚,3组组内3个时相点的相对表达量差异均无统计学意义（P〉0.05）。结论：治伤巴布剂能缓解大鼠炎性疼痛反应,对大鼠炎性疼痛具有一定的镇痛作用,可降低福尔马林足底炎性疼痛大鼠模型背根神经节中辣椒素受体的表达水平。

Impact of Injury Papua Agent on Analgesia and the Expression of Capsaicin Receptor in Model Rats with Formaldehyde-Induced Inflammatory Pain

Objective To investigate the effects of Zhishang cataplasm on the levels of capsaicin receptor in dorsal rootganglion in formalin injected rats, and explore the analgesic effects of Zhishang cataplasm on inflammatory pain. Methods： The36 SD rats were included in the experiment and were randomly divided into blank control group （n=12）, model group （n=12）and treatment group （n=12）. The rats were not given any treatment in blank control group; Rats were given injection of 5% for-malin 0.1ml in the left plantar in model group; and the rats in treatment group were given Zhishang cataplasm as topical medi-cation one day before modeling and injection of 5% formalin 0.1ml in the left plantar, then Zhishang cataplasm as topical med-ication. The pain behavior was observed, according Dubuisson scoring method as a standard for comparison. The segment of L3-6 dorsal root ganglion was taken from rats in a specific time, and the expression levels of capsaicin receptor in rats dorsal rootganglion was detected with Real-time PCR and western blot were used to. Results： The modeling group and treatment groupwere both shown typical biphasic nociceptive behavior reactions, but the credits in pain response in treatment group were lowerthan that of the model group （P〈0.05）. Realtime-PCR showed that the expression of capsaicin receptor in model group was higherthan that of the treatment group, and the expression of capsaicin receptor in treatment group than was higher that of the blankcontrol group, with statistically significant difference （P〈0.05）. In each group, the difference on expression levels of capsaicin receptoramong three time points showed no statistically significant differences （P〉0.05）. Western blot showed that the expression of capsaicinreceptor in model group was higher than that of the treatment group, and the expression of capsaicin receptor in treatment groupthan was higher that of the blank control group, with statistically significant difference （P〈O.05）. In each group, the d