诊断超声照射后人早孕绒毛细胞p53 mRNA bcl-2 mRNA表达改变

目的　探讨诊断超声与人早孕绒毛 p5 3 m RNA和 bcl-2 m RNA表达的关系。方法　对拟进行人工流产的 2 4例早孕 (4 5～ 60 d)妇女随机分为 4组 :对照组、10 m in组、2 0 min组和 3 0 min组。应用 HP-85 0 0彩色多普勒超声诊断仪 ,对孕囊进行不同时间的持续照射 ,照射后 2 4h取材。用原位杂交技术检测上述各组绒毛滋养层细胞 p5 3 m RNA和 bcl-2 m RNA表达情况。结果　超声照射后 10 min组绒毛滋养层细胞 p5 3 m RNA和 bcl-2 m RNA表达率与对照组差异无显著性 (P>0 .0 5 ) ,2 0 min组和 3 0 min组滋养层细胞 p5 3 m RNA表达率明显增加 ,显著大于对照组和 10 min组 (P<0 .0 1) ;而 bcl-2 m RNA表达率却明显下降 ,显著低于对照组和 10 min组 (P<0 .0 1)。结论　诊断超声持续照射早孕孕囊组织 >10 min可引起绒毛滋养层细胞 p5 3 m RNA表达率增加和 bcl-2 m RNA表达率下降 ,从而诱导滋养层细胞凋亡增加。

p53 mRNA and bcl-2 mRNA expression in first-trimester pregnancy′s trophoblast after diagnostic ultrasound irradiation

Objective To investigate the role of p53 mRNA and bcl 2 mRNA expression in first trimester pregnancy′s trophoblast after diagnostic ultrasound irradiation.Methods Twenty four first trimester pregnant women were randomly separated into four groups of six according to different exposure time:control group (blank),group 10 min irradiation,group 20 min,group 30 min.Gestational sacs were irradiated by HP 8500 color Doppler ultrasonography with 3.5 MHz transducer.Their trophoblastic were removed at 24 h after irradiation.Expression of p53 mRNA,bcl 2 mRNA was analyzed with in situ hybridization.Results The positive rate of p53 mRNA in experiment groups except the group 10 min was all higher;but that of bcl 2 mRNA was lower.The rate of p53 mRNA expression in syncytiotrophoblast and cytotrophoblast was (54±6)% and (54±4)% respectively in control group;that of bcl 2 mRNA in syncytiotrophoblast and cytotrophoblast was (70±4)% and (74±7)% respectively in control group.After irradiation the positive rate of p53 mRNA expression was peaking to (60±11)% and (61±11)% in group 20 min and (64±9)% and (67±8)% in group 30 min;that of bcl 2 mRNA expression reached to (61±4)% and (63±5)% in group 20 min respectively.There was no difference between control group and group 10 min (P>0.05).Conclusion Irradiation continuously over 10 min by diagnostic ultrasound may result in higher expression of p53 mRNA and lower expression of bcl 2 mRNA in first trimester pregnancy,further inducing trophoblasts cell to get a raise in apoptosis.