依达拉奉对离体兔肺脏保存效果的实验研究

目的探讨依达拉奉对离体兔肺脏保存质量的影响。方法健康大白兔12只,按照随机原则,分为LPD组(n=6)和E组(n=6)。LPD组用低钾右旋糖酐(LPD)液行肺灌洗及肺保存,E组用含依达拉奉20 mg/L的LPD液行肺灌洗及肺保存。实验结束时取肺静脉血标本作血气分析及制备血清测定丙二醛(MDA)含量与超氧化物歧化酶(SOD)活性;取肺组织标本测干/湿重及送病理。结果两组肺静脉血PaO2、PaCO2、血浆丙二醛(MDA)含量及SOD活性、肺组织干/湿重比(D/W)均无显著差异(P>0.05)。肺组织病理结果显示两组均有肺组织毛细血管轻度充血,肺泡结构尚可,肺泡间质少量粒细胞浸润,肺泡腔内轻度渗出及少量红细胞。结论在离体冷保存6 h再灌注的兔肺模型,依达拉奉未显示出明显优于低钾右旋糖酐液(LPD)的保护效果。

The Effect of Edaravone on Isolated Rabbit Lung Preservation

OBJECTIVE To investigate the pulmonary preservation effect of Edaravone on isolated rabbit lung.METHODS 12 rabbits were randomly divided into 2 groups: LPD group(n=6),lungs flushed and preserved with LPD solution;Edaravone group(n=6): lungs flushed and preserved with LPD solution plus Edaravone 20mg/L.All lungs were reperfused with venous blood for 10 minutes after 6 hours cold preservation.Blood samples were analyzed for PO_2,PCO_2,serum MDA and SOD.Lung tissue wet-to-dry weight ratio compared,samples of left lung tissue were sent for histologic evaluation.RESULTS PaO_2 and PaCO_2 levels,serum MDA and SOD,and Lung tissue D/W ratio showed no significant difference between two groups(P>0.05);histologic evaluation showed mild changes in lung tissue in both groups.CONCLUSION LPD solution plus edaravone and LPD solution showed similar protective effects on isolated rabbit lung preservation.