用于姜黄素光动力治疗的紫光LED光疗仪的研制

目的：设计一款适用于姜黄素光动力治疗的紫光LED光疗仪，并观察用该光疗仪照射姜黄素对人舌鳞状细胞癌Tca8113细胞生长抑制作用。方法：针对姜黄素（CUR）光敏化反应所需特定吸收波长，选择峰值波长为405nm的大功率发光二级管作为光源，采用AD9850生成脉宽调制信号（PWM），该脉宽调制信号可实现占空比的连续调节，PWM送入以MAX5035搭建的恒流驱动电路．以实现光强连续调节。使用该光疗仪对经过姜黄素孵育的Tca8113细胞进行照射，最终采用MTT法检测该光疗仪照射姜黄素对细胞抑制率的影响。结果：该光疗仪输出的最大光功率可达75mW，中心波长为405nm，半峰值宽度为5nm；选择光剂量为1．2J／cm^2和2．4J／cm^2进行经姜黄素2小时孵育细胞的光照实验．姜黄素浓度分别为5μmol／L、10μmol／L、20μmol／L、40μmol／L、80μmol／L，MTT测试结果表明可明显抑制人舌鳞状细胞癌Tca8113细胞的生长，光剂量为1．2J／cm^2组的细胞抑制率分别为12．1％，19．1％，40．9％，68．7％，8l-3％。而2．4J／cm^2组的细胞抑制率分别为18．2％，23．4％，56．6％，77．6％，84．1％。结论：本光疗仪能够实现姜黄素光动力学的细胞抑制作用，并且证明姜黄素在紫光（405±5）nm的波长下有较好的光敏化反应。

Development of a Violet Light-emitting Diode Phototherapy System for Curcumin Photodynamic Therapy

Objective:To design a violet light-emitting diode phototherapy instrument which is applicable for curcumin photodynamic therapy and to investigate the effect of curcumin mediated PDT(CUR-PDT) on human tongue squamous cell carcinoma cell line Tca8113.Methods: According to the specific absorption wavelength needed of curcumin(CUR) photosensitive reaction,our group built a violet light-emitting diode as the light source for CUR-PDT phototherapy System.The AD9850 was used for pulse width modulation(PWM) which could achieve continuously adjustable duty cycle to realize the continuous adjust light intensity.The PWM signal was sent to constant current driver circuit built with MAX5035 to realize the irradiance control.We chose the light dose as 1.2 J/cm2 and 2.4 J/cm2 with the Tca8113 cells of the experiment incubating in various curcumin concentrations(5 μmol/L,10 μmol/L,20 μmol/L,40 μmol/L,80 μmol/L) for 2 hours.MTT assay showed that after the treatment of CUR-PDT,the inhibition of Tca8113 cells was significant.Within the light dose of 1.2 J/cm2,the cells inhibition rates were 12.1%,19.1%,40.9%,68.7%,and 81.3% respectively.Meanwhile,the cell inhibition rates of 2.4 J/cm2 group were 18.2%,23.4%,56.6%,77.6%,and 84.1% respectively.Results: The irradiance of LED instrument can reach 75 mW,with the wavelength 405 nm and 5 nm of half peak width.MTT shows that with the phototherapy instrument we designed,the growth of human tongue squamous cell carcinoma Tca8113 cells can be inhibited obviously.Conclusions: This phototherapy apparatus can realize the curcumin photodynamic dynamics of cells inhibition.Moreover,our study proves that under the wavelength(405±5)nm of light,curcumin has well photosensitive reaction.