尿液特征组分与糖尿病早期肾损害的关系初探

目的对尿液特征组分与糖尿病早期肾损害的关系进行了初步探索。方法对2011年12月-2012年5月间28例2型糖尿病组、33例2型糖尿病肾病组及26例健康对照组尿液中尿蛋白含量和几种常见非蛋白氮物质，包括肌酸、尿囊素、肌酐、尿酸和假尿嘧啶核苷的浓度进行测定，采用多种归一化方法对数据进行对比分析，并通过t检验减少高效液相色谱测定的变量信息，保留P〈O．05的检出峰进行主成分分析，获得分类结果。结果采用体积归一化方法，发现健康对照组尿液中肌酸、尿囊素和尿酸的含量与2型糖尿病组和糖尿病肾病组相比，差异均有统计学意义（P〈0．05），2型糖尿病组尿液中尿蛋白的浓度与糖尿病肾病组相比，差异有统计学意义（P〈0．05）。结论通过肌酸、尿囊素、尿酸和尿蛋白的联合测定为肾脏损伤程度的监测及疗效观察提供依据，为2型糖尿病患者肾功能损坏的早期预防与诊断进行初步判断提供了新的方法。

Research on the Relationship between Urine Components and the Diabetes Early Renal Damage

Objective To research on the relationship between early renal damage of diabetes and components in the urine.Methods From December 2011 to May 2012,29 patients with type 2 diabetes mellitus(T2DM),33 patients with diabetic nephropathy,and 26 healthy controls were included in our study.The concentrations of urine protein(PRO) and five common non-protein nitrogen substances including urine creatinine(Cn),creatine(Cr),uric acid(Ua),allantion(An) and pseudouridine(Pu) were determined.Normalization method was used for data analysis.t-test was used to decrease the variable information detected by high performance liquid chromatography.The peak detected when P was under 0.05 was retained for main components analysis and assorted results.Results The volume normalization method was used.The content of Cr,An and Ua showed significant differences(P < 0.05) between healthy controls and T2DM patients,and between healthy controls and patients with diabetic nephropathy.The concentration of urine protein showed significant difference(P < 0.05) between T2DM patients and patients with diabetic nephropathy.Conclusions The combined determination of the contents of Cr,An,Ua,PRO/Cr and PRO/Pu was found to be sensitive index for monitoring early kidney damage and predicting the clinical outcomes.It can provide early diagnosis and preliminary judgment for T2DM patients with renal damage.