广西麻鸭乙型肝炎感染病毒动物模型的实验研究

目的探讨广西麻鸭作为乙型肝炎病毒感染动物模型的可能性。方法应用广西1 d龄雏麻鸭经腹腔感染鸭乙型肝炎病毒(DHBV),13 d后采用实时荧光定量PCR法检测麻鸭血清DHBV含量,筛选出DHBV强阳性鸭。结果共检测148份麻鸭血清标本,其中DHBV阳性标本136份,阳性率为91.9%。结论广西麻鸭可作为乙型肝炎病毒感染动物模型。实时荧光定量PCR法检测DHBV敏感性较高,重复性好,可用于DHBV检测。

Experimental study of guangxi brown spotted duck as hepatitis B virus infection animal model

Objective To explore the possibility of Guangxi brown spotted duck as hepatitis B virus infection animal model.Methods One-day old Guangxi brown spotted ducks were infected with duck hepatitis B virus(DHBV), and DHBV strong positive ducks were detected by the real-time fluorescence quantitative PCR(FQ-PCR) at 13 day after the infection of DHBV.Results One hundred and forty-eight serum samples in ducks were tested,and 136 samples(91.9%) were positive for DHBV.Conclusion The Guangxi brown spotted duck may be used as hepatitis B virus infection animal model.FQ-PCR method for detection of DHBV is sensitive,repeatable and practicable.