天麻成分抗花生四烯酸诱导的家兔体外血小板聚集活性研究

目的:观察天麻成分抗花生四烯酸(Arachidonic Acid,AA)诱导的家兔体外血小板聚集作用,明确其作用物质基础。方法:以天麻乙酸乙酯部位分离提取得到的B、C、D成分以及天麻素为研究对象,采用比浊法测定4个成分抗AA诱导的家兔体外血小板聚集作用。结果:天麻B、C、D成分高浓度(0.4mg.mL-1)对AA诱导的家兔血小板聚集均有抑制作用,IC50分别为0.104,0.163,0.263mg.mL-1,以天麻B成分抑制作用较强,并呈现一定的浓度依赖。天麻素5个浓度组与对照组比,对AA诱导的家兔体外血小板聚集的聚集率差异均无显著性意义。结论:天麻B、C、D成分均具有一定的抗AA诱导的家兔体外血小板聚集作用,以B成分抑制作用较强。在本研究浓度下,天麻素作用不明显。

The Study of the Anti-platelets Aggregation Effect of Compounds from Gastrodia Induced by AA in Vitro

Objective ： To observe the effects of the compounds from gastrodia on anti - platelets aggrega- tion induced by AA in vitro and define its material base. Methods ： Anti - platelets aggregation effects of B, C, D separated from the ethyl acetate extracted parts of Gastrodia elata Blume and gastrodin were observed by light trans- mission in vitro. AA was used as inducers. Results： Compounds B, C, D all had some effects on anti- platelets aggregation at high concentration （0. 4 mg ·mL^-1 ）, ICs0 is 0. 104, 0. 163, 0. 263mg ·mL^-1. The inhibition of compound B was much stronger and presented certain concentration dependence. Gastrodin had no significant effects on rabbit blood platelets aggregation induced by AA in vitro. Conclusion： Compounds B, C, D all had some effects on anti -platelets aggregation, among which, compound B was much stronger. Gastrodin had no sig- nificant effects at the same concentration.