基于小鼠生理生化指标探讨生半夏和竹沥半夏的寒热药性

目的:通过对小鼠的生理生化指标的检测分析,探讨生半夏和竹沥半夏的寒热药性。方法:采用灌胃分别给予小鼠生半夏、竹沥半夏的水煎液11d,测量并记录小鼠的体重、饮水量、进食量、体温、耗氧量、肝组织总蛋白含量、NaK-ATPase活性、肝脏系数的变化,来研究其寒热之药性。结果:与对照组比较,生半夏组、竹沥半夏组对小鼠的体重、进食量、肝脏系数的变化均无明显区别;生半夏组可使小鼠的饮水量、体温、耗氧量、NaK-ATPase活力、肝脏组织总蛋白含量增加;竹沥半夏组可使小鼠的饮水量、体温、耗氧量、NaK-ATPase活力、肝脏组织的蛋白含量降低。结论:生半夏和竹沥半夏药性的差异可通过饮水量、体温、耗氧量、NaK-ATPase活力、肝脏组织总蛋白含量等生理生化指标得以反映,该结果与传统的"竹沥能转半夏之温热之性而为寒凉"的理论相一致。

Evaluation on Cold or Heat Nature Based on Physiological and Biochemical Characteristics of Unprocessed Rhizoma Pinelliae and Rhizoma Pinelliae with Bamboo Vinegar Processed

Objective： Testing the physiological and biochemical parameters in mice, to evaluate the na- ture of unprocessed Rhizoma Pinelliae （RP） and Rhizoma Pinelliae With Bamboo Vinegar Processed （RP- BVP）. Methods： Measured weight, water intake, food intake, body temperature, oxygen consumption, total pro- tein content of liver tissue, Na - K - ATPase activity, liver coefficient of mice after giving the water decocted solu- tion of RP and RP - BVP intragestrically 11 days. Results ： Compared with control group, weight, food intake, liv- er coefficient had no significant difference in RP and RP - BVP group ; Compared with control group, water intake, body temperature, oxygen consumption, Na - K - ATPase activity, total protein content of liver tissue increased in RP group; oxygen consumption, the liver tissue total protein content, Na - K - ATPase activity reduced in RP - BVP group. Conclusion ： water intake, body temperature, oxygen consumption, Na - K - ATPase activity, total protein content of liver tissue can be used to evaluate the cold and heat nature of RP and RP - BVP.