| 雷公藤内酯醇体内外对HL-60细胞作用的研究 |
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| 引用本文： | 俞萍丽,陈元仲,许建华,吴勇,陈显凌. 雷公藤内酯醇体内外对HL-60细胞作用的研究[J]. 中国药理学通报, 2007, 23(10): 1346-1348 |
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| 作者姓名： | 俞萍丽 陈元仲 许建华 吴勇 陈显凌 |
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| 作者单位： | 1. 福建医科大学附属协和医院,福建省血液病研究所,福建,福州,350001
2. 福建医科大学药学院,福建,福州,350004 |
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| 基金项目： | 福建省重点建设高校资助项目;福建省重大专项前期研究课题 |
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| 摘 要： | 目的探讨雷公藤内酯醇(triptolide,TPL)在体内外对人髓系白血病细胞株HL-60的作用。方法运用MTT比色法检测TPL对HL-60细胞的增殖抑制作用,采用TdT酶介导的缺口末端标记法(TUNEL)检测TPL对HL-60细胞的诱导凋亡作用;建立HL-60裸鼠异种移植瘤模型,观察经TPL治疗后的肿瘤抑制率。结果在体外,TPL对HL-60细胞的增殖具有明显的抑制及诱导凋亡作用,呈时间和剂量依赖性;在体内,TPL可抑制HL-60细胞裸鼠异种移植瘤生长,抑制率最高可达53.5%。结论TPL在体内外均可抑制HL-60细胞增殖,并诱导其凋亡。
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| 关 键 词： | 雷公藤内酯醇 HL-60细胞 细胞凋亡 HL-60细胞裸鼠移植瘤 |
| 文章编号： | 1001-1978（2007）10-1346-03 |
| 修稿时间： | 2007-06-09 |
Effects of Triptolide on HL-60 Cell in vitro and in vivo |
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| YU Ping-li,CHEN Yuan-zhong,XU Jian-hua,WU Yong,CHEN Xian-ling. Effects of Triptolide on HL-60 Cell in vitro and in vivo[J]. Chinese Pharmacological Bulletin, 2007, 23(10): 1346-1348 |
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| Authors: | YU Ping-li CHEN Yuan-zhong XU Jian-hua WU Yong CHEN Xian-ling |
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| Affiliation: | 1. Fujian Institute of Hematology; Union Hospital, Fujian Medical University, Fuzhou 350001, China; 2. College of Pharmacy, Fujian Medical University, Fuzhou 350004, China |
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| Abstract: | Aim To study the effects of Triptolide (TPL) on HL-60 cells in vitro and in vivo.Methods MTT was used to examine the effects of TPL on proliferation of HL-60 cells;TUNEL assay was used to detect apoptotic cells.The effect of TPL on xenograft growth of HL-60 cells was evaluated by tumor inhibition rate.Results In vitro TPL inhibited the proliferation and induced apoptosis of HL-60 cells in a dose-dependent manner.In vivo TPL inhibited xenograft growth of HL-60 cells with the highest inhibition rate of 53.5%.Conclusion TPL is able to inhibit the proliferation of HL-60 cells and induce apoptosis of HL-60 cells in vitro and in vivo. |
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| Keywords: | triptolide HL-60 apoptosis xenograft HL-60 cells in nude mice |
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