葛花提取工艺研究

目的：优选葛花的最佳提取工艺。方法：提取工艺研究采用正交试验法，筛选出葛花药材的最佳提Jgx-艺。采用HPLC法测定葛花有效成分鸢尾苷的含量。色谱条件：色谱柱：DiamosilC18（2）（150mm×4．6mm，5μm）；柱温：室温；流动相：乙腈-水（20：80）；波长：265nln；流速：1．0ml·min^-1；柱温：室温。结果：葛花的最佳提取工艺为药材加入40％乙醇溶液提取2次，第1次14倍量提取1．5h，第2次12倍量提取1．0h。鸢尾苷浓度在1．1～30μg·ml^-1。之间与峰面积线性关系良好，r=0．9999；鸢尾苷平均回收率为100．3％，RSD=2．0％。结论：本试验方法简单，专属性强，重复性好。

Study on Extraction Process and Content Determination of Flos Puerariae

To optimize the extracting condition of tectoridin in Flos Puerariae and determine the content. Method： The extracting process was optimized by orthogonal design. The content of tectoridin in Flos Puerariae was measured by HPLC with the following conditions：the chromatographic column was Diamosil Cls （2）（ 150 mm× 4. 6 mm,5μm） with detection wavelength of 265 nm, the mobile phase was acetonitrile-water （20： g0） with the flow rate of 1.0ml·min^- 1, and the column temperature was ambient. Result： The optimal extrating conditions were the following：Flos Puerariae was recirculated with 40% ethonal for 2 times ,the first time was with 14-fold amount of Flos Puerariae for 1.5 h,and the second time with 12-fold amount of Flos Puerariae for 1.0 h. The average recovery of tectoridin was 100. 3% with RSD of 2. 0%. Conclusion： The studies show that the methods are sufficiently simple, sensitive and re- producible.