HPLC法测定枳实导滞丸中芦荟大黄素、大黄酸、大黄素、大黄酚及大黄素甲醚的含量

目的:建立枳实导滞丸中芦荟大黄素、大黄酸、大黄素、大黄酚及大黄素甲醚的含量测定方法。方法:采用HPLC法,色谱柱:Waters Symmery C₁₈（250mm×4.6mm,5μm）,流动相:甲醇-0.1%磷酸水溶液（85:15）,检测波长:254nm,流速:1.0ml·ml^-1。结果:芦荟大黄素、大黄酸、大黄素、大黄酚及大黄素甲醚的线性范围分别为0.011～0.226μg（r=0.9998）、0.005～0.092μg（r=0.9999）、0.010～0.194μg（r=0.999 9）、0.011～0.212μg（r=0.9999）、0.005～0.102μg（r=0.9998）;浓度范围内进样量与峰面积线性关系良好。平均回收率分别为97.47%（RSD=1.56%）、99.57%（RSD=0.91%）、100.66%（RSD=1.09%）、101.97%（RSD=1.60%）、101.54%（RSD=1.58%）（n=6）。结论:所建方法简单、快速、准确,可用于枳实导滞丸的质量控制。

Determination of Aloe-emodin, Rhein, Emodin, Chrysophanol and Physcion in Zhishi Daozhi Pills by HPLC

To establish a method for the determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in Zhishi Daozhi pills. Method： The separation was performed on waters Symmery C18（250 mm × 4.6 ram,5μm） column with detection wavelength of 254 nm. The mobile phase consisted of methanol-0.1% phosphoric acid solution （85：15 ） with the flow rate of 1.0 ml·ml^-1 Result： The linear range of aloe-emodin, rhein, emodin, ehrysophanol and physeion was within 0. 011-0. 226μg （ r = 0. 999 8 ）, 0. 005 -0. 092 μg （ r = 0. 999 9 ）, 0. 010 -0. 194 μg （ r = 0. 999 9 ）, 0.011-0. 212 μg （ r = 0. 999 9 ） and 0. 005-0. 102μg （ r = 0. 999 8 ） with the average recovery of 97. 47% （ RSD = 1.56% ）, 99. 57% （ RSD = 0. 91% ）, 100. 66% （ RSD = 1.09% ）, 101.97% （ RSD = 1.60% ） and 101.54% （RSD = 1.58% ） ,respectively （n =6）. Conclusion： The method is simple ,rapid and accurate. It can be used for the quality control of Zhishi Daozhi pills.