| HPLC法测定复方氯霉素阴道泡腾片中己烯雌酚的含量 |
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| 引用本文： | 郭江红,赵亚萍.HPLC法测定复方氯霉素阴道泡腾片中己烯雌酚的含量[J].中国药师,2011,14(6):825-826. |
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| 作者姓名： | 郭江红 赵亚萍 |
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| 作者单位： | 湖北省食品药品监督检验研究院,武汉,430064 |
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| 摘 要： | 目的:建立高效液相色谱法测定复方氯霉素阴道泡腾片中己烯雌酚的含量。方法:色谱柱为DIONEX C18（250mm×4.6mm,5μm）;流动相为甲醇-水（75:25）;流速为1.0 ml·min-1;检测波长为239nm;进样量为50μl。结果:己烯雌酚的线性范围为1.1～8.6μg·ml-1（r=0.9999）;平均回收率为100.8%（RSD=1.9%）。结论:本方法结果准确可靠,可作为复方氯霉素阴道泡腾片中己烯雌酚的含量测定方法。
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| 关 键 词： | 高效液相色谱法 复方氯霉素阴道泡腾片 己烯雌酚 含量测定 |
Determination of Diethylstiibestrol in Compound Chloramphenicol Vaginal Effervescent Tablets by HPLC |
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| Guo Jianghong,Zhao Yaping.Determination of Diethylstiibestrol in Compound Chloramphenicol Vaginal Effervescent Tablets by HPLC[J].China Pharmacist,2011,14(6):825-826. |
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| Authors: | Guo Jianghong Zhao Yaping |
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| Institution: | Guo Jianghong,Zhao Yaping(Hubei Institute for Food and Drug Control,Wuhan 430064,China) |
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| Abstract: | Objective： To establish an HPLC method for determination of diethylstilbestrol in compound chloramphenicol vaginal effervescent tablets. Method： The column was DIONEX C18 （250 mm ×4. 6 ram, 5 μm）, the mobile phase consisted of methanol- water （75： 25） at the flow rate of 1.0 ml·min^-1 , the detection wavelength was 239 nm and the injection volume was 50 μl. Result： The linear range of diethylstilbestrol was 1.1-8. 6 μg· ml^-1（ r= 0. 999 9）, and the average recovery was 100. 8% （RSD = 1. 9% ） o Conclusion： This method is accurate and suitable for determination of diethylstilbestrol in compound chloramphenicol vaginal effervescent tablets. |
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| Keywords: | HPLC Compound chloramphenicol vaginal effervescent tablets Diethylstilbestrol Determination |
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