| 蛛网膜下腔出血后血管痉挛与S100b基因表达的相关性 |
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| 引用本文： | 谷志龙,杨福义,杨卫东,张瑶.蛛网膜下腔出血后血管痉挛与S100b基因表达的相关性[J].黑龙江医药科学,2011,34(3):15-16. |
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| 作者姓名： | 谷志龙 杨福义 杨卫东 张瑶 |
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| 作者单位： | 佳木斯大学附属第一医院神经外科,黑龙江,佳木斯,154003 |
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| 摘 要： | 目的：观察蛛网膜下腔出血（SAH）模型大鼠基底动脉中S100b基因表达的情况,探讨S100b与SAH后血管痉挛（CVS）的相关性。方法：健康雄性Wistar大鼠30只,随机分为对照组、假手术组和模型组。将模型组大鼠断尾取血0.3mL经环枕膜注入枕大池,假手术组以同样方法注入等量生理盐水。3d后断髓处死取基底动脉,通过HE染色观察基底动脉的病理变化;采用RT-PC法检测S100b mRNA表达量,蛋白质印迹（Western Blot）染色法测定S100b蛋白含量。结果：模型组大鼠基底动脉的管腔明显变小,对照组和假手术组无明显改变;S100b在对照组和假手术组都有少量表达,但两组无显著性差异（P〉0.05）;与假手术组相比,模型组大鼠基底动脉中S100b mRNA及蛋白的表达显著增高（P〈0.01）。结论：蛛网膜下腔出血后S100b基因表达显著增高,并且和血管痉挛的发生成正相关。
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| 关 键 词： | 蛛网膜下腔出血 血管痉挛 S100b基因 |
Correlation between expression of S100b gene and CVS after SAH |
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| GU Zhi-long,YANG Fu-yi,YANG Wei-dong,ZHANG Yao.Correlation between expression of S100b gene and CVS after SAH[J].Heilongjiang Medicine and Pharmacy,2011,34(3):15-16. |
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| Authors: | GU Zhi-long YANG Fu-yi YANG Wei-dong ZHANG Yao |
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| Institution: | GU Zhi-long,YANG Fu-yi,YANG Wei-dong,ZHANG Yao(The First Affiliated Hospital of Jiamusi University,Jiamusi 154003,China) |
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| Abstract: | Objective:To observe the expression of S100b gene in basal artery from subarachnoid hemorrhage(SAH) rat models and discuss the relevance between S100b with vasospasm(CVS) after SAH.Methods:Thirty healthy Wistar rats were divided into three groups randomly: control group,sham operation group and operation model group.The subarachnoid hemorrhage model was induced by transorbital injection of 0.3mL of nonheparinized autologous arterial blood into the chiasmatic cistern and the sham operation group was injected... |
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| Keywords: | subarachnoid hemorrhage vasospasm S100b gene |
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