| LRG介导内毒素预处理诱导的脑保护作用 |
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| 作者姓名： | Gong G Huang Y Yuan LB Hu L Cai L |
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| 作者单位： | 1. 成都军区总医院麻醉科,四川成都,610083
2. 第四军医大学西京医院麻醉科,陕西西安,710032 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的:通过测定不同组大鼠脑缺血/再灌注损伤模型中脂多糖应答分子(LRG)表达水平的变化与炎症因子含量及大鼠脑梗死容积之间的关系,探讨内毒素重复预处理诱导脑保护效应的机制。方法:将66只SD大鼠随机分为假手术组、缺血对照组和内毒素预处理组,每组22只。采用戊巴比妥钠(40 mg/kg)腹腔注射麻醉大鼠,采用大鼠大脑中动脉栓塞模型(MCAO)制备大鼠大脑缺血/再灌注模型。采用免疫印迹法结合图像分析软件半定量计算LRG分子表达水平的变化,酶联免疫法测定脑组织TNF-α、IL-1β、IL-6、IL-10的含量,用氯化三苯四唑(TTC)染色法和计算机图像分析系统计算大鼠大脑梗死容积。结果:相比于缺血对照组,内毒素重复预处理组大鼠大脑LRG分子表达水平明显上调,TNF-α、IL-1β、IL-6含量明显降低,IL-10的含量明显增高,同时,内毒素预处理组较缺血对照组神经功能评分明显升高,脑梗死容积明显缩小。结论:内毒素重复预处理通过上调LRG分子表达,促进致炎-抑炎因子之间的动态平衡介导脑保护作用。
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| 关 键 词： | 内毒素脂多糖 脂多糖应答分子 脑缺血再灌注损伤 炎症因子 |
Requirement of LRG in endotoxin-mediated brain protection |
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| Gong G,Huang Y,Yuan LB,Hu L,Cai L.Requirement of LRG in endotoxin-mediated brain protection[J].Journal of Cellular and Molecular Immunology,2011,27(8):865-867. |
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| Authors: | Gong Gu Huang Yi Yuan Li-bang Hu Ling Cai Lin |
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| Institution: | GONG Gu1,HUANG Yi2,YUAN Li-bang2,HU Ling1,CAI Lin11Department of Anesthesiology,General Hospital of People's Liberation Army Chengdu Military Region,Chengdu 610083,2Department of Anesthesiology,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,China |
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| Abstract: | AIM: To explore the protective mechanism of LPS pretreatment in a rat model of cerebral ischemia-reperfusion injury.METHODS: Sixty-six male Spraque-Dawley rats were randomly divided into a sham group(n=22),a control group(n=22) and a LPS group(n=22).The control group and the LPS group were subjected to focal cerebral isehmia induced by middle cerebral artery occlusion(MCAO) for 2 hours.The LPS group was pretreated with LPS for five consecutive days with the last pretreatment at 24 hours before the surgery,w... |
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| Keywords: | LPS LRG cerebral ischemia-reperfusion injury inflammatory factor |
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