HPLC法同时测定口腔溃疡液中3组分的含量

目的:建立HPLC法同时测定口腔溃疡液中氯霉素、醋酸地塞米松及盐酸丁卡因等3组分的含量。方法:色谱柱采用Kromasil C₁₈柱（250mm×4.6mm,5μm）,甲醇-水-乙腈（45:45:10）（含0.02%庚烷磺酸钠,0.34%磷酸二氢钾,用三乙胺调pH至6.5）为流动相。检测波长240～340nm。结果:氯霉素在8.527-25.581mg·ml^-1（r=0.9992）,醋酸地塞米松在0.127～0.381mg·ml^-1（r=0.999 9）,盐酸丁卡因在5.014～15.019mg·ml^-1（r=0.9992）浓度范围内线性关系良好。氯霉素的平均回收率为99.16%,RSD为0.47%;醋酸地塞米松的平均回收率为99.03%,RSD为0.62%;盐酸丁卡因的平均回收率为100.40%,RSD为1.16%。结论:该方法具有操作简便,结果准确的优点,可用于口腔溃疡液中的氯霉素、醋酸地塞米松及盐酸丁卡因的质量控制。

Simultaneous Determination of Three Components in Oral Ulcer Liquid by HPLC

Objective： To establish an HPLC method for simultaneous determination of three components in oral ulcer liquid containing chloramphenicol, dexamethasone acetate and tetracaine hydrochloride. Method： A Kromasil C18 column （250 mm× 4. 6 mm, 5 μm） was used. Methanol - water - acetonitrile （45： 45： 10） （containing 0. 02% sodium heptanc and 0. 34% potassium dihydrogen phosphate, and adjusted pH to 6. 5 with triethylamine ） was employed as the mobile phase. Detection wavelength was set at 240-340 nm. Result： Within the range of 8. 527-25. 581 mg·ml^-1, 0. 127-0. 381 mg·ml^-1 and 5. 014-15. 019 mg·ml^-1 for chloramphenicol, dexamethasone acetate and tetracaine hydrochloride, good linear relationships were obtained with r of 0. 999 2, 0. 999 9 and 0. 999 2, respectively. The average recoveries of chloramphenicol, dexamethasone acetate and tetracaine hydrochloride were 99. 16% （RSD = O. 47% ） ,99. 03% （RSD = 0. 62% ） , and 100.40% （ RSD = 1.16% ）, respectively. Conclusion： The method is simple and accurate, and can be used for the quality control of oral ulcer liquid.